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Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-small Cell Lung Cancer Cells

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Autophagy in Differentiation and Tissue Maintenance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1854))

Abstract

Autophagy is increasingly identified as a central player in many cellular activities from cell proliferation to cell division, migration, and differentiation. However, it is also considered as a double-edged sword in cancer biology which either promotes oncogenesis/invasion or sensitizes the tumor cells to chemotherapy induced apoptosis. Recent investigations have provided direct evidence for regulation of cellular phenotype via autophagy pathway. One of the most important types of phenotype conversion is Epithelial-Mesenchymal-Transition (EMT), resulting in alteration of epithelial cell properties to a more mesenchymal form. In the current chapter, we provide a method which is established and being used in our laboratory for detection of autophagy and EMT in lung epithelial cells and show the involvement of autophagy in modulation of cellular phenotype.

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Acknowledgement

SG was supported by Health Science Centre Foundation General Operating Grant and University Collaborative Research Program. SS was supported by Health Science Centre Foundation General Operating Grant and University Collaborative Research Program and Mitacs Accelerate Postdoctoral Fellowship. JA was supported by Research Manitoba studentship award.

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Authors and Affiliations

  1. Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada, R3T 2N2

    Javad Alizadeh, Shahla Shojaei & Saeid Ghavami

  2. Department of Immunology, School of Medicine, Jiroft University of Medical Science, Jiroft, Iran

    Adel Sepanjnia

  3. Department of Clinical Biochemistry, Zahedan University of Medical Sciences, Zahedan, Iran

    Mohammad Hashemi

  4. Department of Physiology and Pathophysiology, Regenerative Medicine, Program and Spinal Cord research Center, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada, R3T 2N2

    Eftekhar Eftekharpour

  5. Biology of Breathing Theme, Children’s Hospital Research Institute of Manitoba, University of Manitoba, Winnipeg, MB, Canada, R3T 2N2

    Saeid Ghavami

  6. Health Policy Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran

    Saeid Ghavami

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  1. Javad Alizadeh
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  2. Shahla Shojaei
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  3. Adel Sepanjnia
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  4. Mohammad Hashemi
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  5. Eftekhar Eftekharpour
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  6. Saeid Ghavami
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Correspondence to Saeid Ghavami .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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Alizadeh, J., Shojaei, S., Sepanjnia, A., Hashemi, M., Eftekharpour, E., Ghavami, S. (2017). Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-small Cell Lung Cancer Cells. In: Turksen, K. (eds) Autophagy in Differentiation and Tissue Maintenance. Methods in Molecular Biology, vol 1854. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_84

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  • DOI: https://doi.org/10.1007/7651_2017_84

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