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MALDI Imaging Mass Spectrometry of N-glycans and Tryptic Peptides from the Same Formalin-Fixed, Paraffin-Embedded Tissue Section

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Book cover Tissue Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1788))

Abstract

Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a unique and well developed tool for probing the protein content of formalin-fixed, paraffin-embedded tissue (FFPE). Integral to this approach is the application of trypsin, and more recently peptide N-glycosidase F, to release tryptic peptides or N-glycans from tissue and report localization of distinct species. This is typically done on serial or adjacent tissue sections, and there is an emerging need to understand the colocalized protein population linked to the exact same regions of N-glycans. Here we describe an approach where N-glycans are first imaged from a tissue section followed by reprocessing of the same tissue section for tryptic peptide MALDI IMS. Strategies for colocalizing peptides to target N-glycans or N-glycan regions are described.

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Acknowledgments

This work was supported by the National Institute of Health/National Cancer Institute R21 CA185799 to RRD. PMA appreciates support from the National Institutions of Health through the National Institute of General Medical Sciences P20GM103542.

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Authors and Affiliations

  1. Department of Cell and Molecular Pharmacology, Medical University of South Carolina, 173 Ashley Avenue, BSB358, Charleston, SC, 29425, USA

    Peggi M. Angel, Anand Mehta, Kim Norris-Caneda & Richard R. Drake

Authors
  1. Peggi M. Angel
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  2. Anand Mehta
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  3. Kim Norris-Caneda
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  4. Richard R. Drake
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Corresponding author

Correspondence to Peggi M. Angel .

Editor information

Editors and Affiliations

  1. Professor, Department of Surgery Medicine and Pediatrics Director, Kidney-Pancreas Transplant Program, University of California San Francisco, San Francisco, California, USA

    Minnie M. Sarwal

  2. Asst. Professor, Department of Surgery, University of California San Francisco, San Francisco, California, USA

    Tara K. Sigdel

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© 2017 Springer Science+Business Media New York

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Angel, P.M., Mehta, A., Norris-Caneda, K., Drake, R.R. (2017). MALDI Imaging Mass Spectrometry of N-glycans and Tryptic Peptides from the Same Formalin-Fixed, Paraffin-Embedded Tissue Section. In: Sarwal, M., Sigdel, T. (eds) Tissue Proteomics. Methods in Molecular Biology, vol 1788. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_81

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  • DOI: https://doi.org/10.1007/7651_2017_81

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7852-6

  • Online ISBN: 978-1-4939-7854-0

  • eBook Packages: Springer Protocols

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