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GeLC-MS: A Sample Preparation Method for Proteomics Analysis of Minimal Amount of Tissue

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Tissue Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1788))

Abstract

Application of various proteomics methodologies have been implemented for the global and targeted proteome analysis of many different types of biological samples such as tissue, urine, plasma, serum, blood, and cell lines. Among the aforementioned biological samples, tissue has an exceptional role into clinical research and practice. Disease initiation and progression is usually located at the tissue level of different organs, making the analysis of this material very important for the understanding of the disease pathophysiology. Despite the significant advances in the mass spectrometry instrumentation, tissue proteomics still faces several challenges mainly due to increased sample complexity and heterogeneity. However, the most prominent challenge is attributed to the invasive procedure of tissue sampling which restricts the availability of fresh frozen tissue to minimal amounts and limited number of samples. Application of GeLC-MS sample preparation protocol for tissue proteomics analysis can greatly facilitate making up for these difficulties. In this chapter, a step by step guide for the proteomics analysis of minute amounts of tissue samples using the GeLC-MS sample preparation protocol, as applied by our group in the analysis of multiple different types of tissues (vessels, kidney, bladder, prostate, heart) is provided.

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Acknowledgments

Supported in part by FP7 SysVasc (grant number: 603288).

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Authors and Affiliations

  1. Biotechnology Division, Biomedical Research Foundation, Academy of Athens (BRFAA), Soranou Efesiou 4, Athens, 11527, Greece

    Manousos Makridakis & Antonia Vlahou

Authors
  1. Manousos Makridakis
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  2. Antonia Vlahou
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Corresponding author

Correspondence to Manousos Makridakis .

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Editors and Affiliations

  1. Professor, Department of Surgery Medicine and Pediatrics Director, Kidney-Pancreas Transplant Program, University of California San Francisco, San Francisco, California, USA

    Minnie M. Sarwal

  2. Asst. Professor, Department of Surgery, University of California San Francisco, San Francisco, California, USA

    Tara K. Sigdel

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© 2017 Springer Science+Business Media New York

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Makridakis, M., Vlahou, A. (2017). GeLC-MS: A Sample Preparation Method for Proteomics Analysis of Minimal Amount of Tissue. In: Sarwal, M., Sigdel, T. (eds) Tissue Proteomics. Methods in Molecular Biology, vol 1788. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_76

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  • DOI: https://doi.org/10.1007/7651_2017_76

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7852-6

  • Online ISBN: 978-1-4939-7854-0

  • eBook Packages: Springer Protocols

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