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Identifying Clinically Relevant Proteins for Targeted Analysis in the Development of a Multiplexed Proteomic Biomarker Assay

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Tissue Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1788))

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Abstract

In recent years, hundreds of candidate protein biomarkers have been identified using discovery-based proteomics. Despite the large number of candidate biomarkers, few proteins advance to clinical validation. Here, we describe a hypothesis driven approach to identify candidate biomarkers, previously characterized in the literature, with the highest probability of clinical applicability. A ranking method, the hypothesis directed biomarker ranking (HDBR) system, was developed to score candidate biomarkers based on seven criteria deemed important in the selection of clinically useful biomarkers. The HDBR system was initially applied to identify candidate biomarkers for the development of a diagnostic test for the early detection of colorectal cancer, but this system can be widely applied to identify biomarkers of relevance in different disease states, for diagnosis, prognostication, or any other specific purpose.

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Acknowledgment

The authors have declared no conflict of interest.

Author information

Authors and Affiliations

  1. Cumming School of Medicine, University of Calgary, Calgary, AB, Canada

    Emily M. Mackay & Oliver F. Bathe

  2. Department of Surgery, University of Calgary, Calgary, AB, Canada, T2N 4N2

    Oliver F. Bathe

  3. Division of Surgical Oncology, Department of Oncology, Tom Baker Cancer Centre, University of Calgary, 1331 – 29 Street NW, Calgary, AB, Canada, T2N 4N2

    Oliver F. Bathe

Authors
  1. Emily M. Mackay
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  2. Oliver F. Bathe
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Corresponding author

Correspondence to Oliver F. Bathe .

Editor information

Editors and Affiliations

  1. Professor, Department of Surgery Medicine and Pediatrics Director, Kidney-Pancreas Transplant Program, University of California San Francisco, San Francisco, California, USA

    Minnie M. Sarwal

  2. Asst. Professor, Department of Surgery, University of California San Francisco, San Francisco, California, USA

    Tara K. Sigdel

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Mackay, E.M., Bathe, O.F. (2017). Identifying Clinically Relevant Proteins for Targeted Analysis in the Development of a Multiplexed Proteomic Biomarker Assay. In: Sarwal, M., Sigdel, T. (eds) Tissue Proteomics. Methods in Molecular Biology, vol 1788. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_75

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  • DOI: https://doi.org/10.1007/7651_2017_75

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7852-6

  • Online ISBN: 978-1-4939-7854-0

  • eBook Packages: Springer Protocols

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