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Tissue Proteomics pp 113–121Cite as

Untargeted Screening of Urinary Peptides Using Offline Nano-Liquid Chromatography: MALDI-TOF/TOF Mass Spectrometry

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1788))

Abstract

In renal transplantation, the discovery of early urine biomarkers of graft lesions would be useful in helping physicians to improve patient care and minimize the use of invasive techniques such as biopsies. Over the last years, high-resolution mass spectrometry has been used extensively for the search of biomarkers in various biological fluids. Here we describe a procedure based on reverse-phase nano-HPLC, offline plate spotting, and MALDI-TOF and TOF/TOF applied in our laboratory for the search of natural peptides in urine samples from renal transplant patients.

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Authors and Affiliations

  1. Department of Pharmacology-Toxicology-Pharmacovigilance, INSERM UMR 850, CHU Limoges, University of Limoges, 2 Avenue Martin-Luther King, Limoges Cedex, 87042, France

    François-Ludovic Sauvage, Sébastien Passeron & Pierre Marquet

  2. U850 INSERM, University of Limoges, CHU Limoges, F-87000, Limoges Cedex, France

    François-Ludovic Sauvage, Sébastien Passeron & Pierre Marquet

Authors
  1. François-Ludovic Sauvage
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  2. Sébastien Passeron
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  3. Pierre Marquet
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Corresponding author

Correspondence to Pierre Marquet .

Editor information

Editors and Affiliations

  1. Professor, Department of Surgery Medicine and Pediatrics Director, Kidney-Pancreas Transplant Program, University of California San Francisco, San Francisco, California, USA

    Minnie M. Sarwal

  2. Asst. Professor, Department of Surgery, University of California San Francisco, San Francisco, California, USA

    Tara K. Sigdel

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Sauvage, FL., Passeron, S., Marquet, P. (2017). Untargeted Screening of Urinary Peptides Using Offline Nano-Liquid Chromatography: MALDI-TOF/TOF Mass Spectrometry. In: Sarwal, M., Sigdel, T. (eds) Tissue Proteomics. Methods in Molecular Biology, vol 1788. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_74

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  • DOI: https://doi.org/10.1007/7651_2017_74

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7852-6

  • Online ISBN: 978-1-4939-7854-0

  • eBook Packages: Springer Protocols

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