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Glutaraldehyde Cross-linking Modification of Decellularized Rat Kidney Scaffolds

Protocol
Part of the Methods in Molecular Biology book series

Abstract

The decellularized (DC) scaffolds retain three-dimensional structures for the stimulation of cell growth, with components of the extracellular matrix (ECM) relatively conserved between species. The strategy based on decellularized scaffolds provides a new way for organ regeneration, with a number of prominent advances having been reported in the past few years. While their lack of biomechanical strength and excessive degradation limit the clinical applications, therefore it is urgent to modify the DC scaffolds to improve the performance.

In this article we describe a simple and robust modification protocol for DC rat kidney scaffolds. To modify, we perfuse DC rat kidneys with glutaraldehyde through the perfusion circulation of the decellularization. After cross-linking, kidney scaffolds are harvested for evaluation of histology, structural stability, and biocompatibility, involving water absorption testing, biomechanical testing, scanning electron microscopy, and several different histological and immunofluorescent analyses.

Keywords

Cross-linking Decellularized scaffolds Degradation Kidney Tissue/organ engineering 

Notes

Acknowledgments

This research was supported by National Natural Science Foundation of China (81570608).

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of Orthopedic SurgeryShanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghaiChina
  2. 2.Department of Plastic SurgeryAffiliated Hospital of Zunyi Medical CollegeZunyiChina
  3. 3.Anatomy DepartmentWenzhou Medical UniversityWenzhouChina

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