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Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells

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Autophagy in Differentiation and Tissue Maintenance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1854))

Abstract

The Caco-2 colorectal cancer cell line is widely used as a model for intestinal differentiation and barrier function. These cells, upon reaching confluency, spontaneously differentiate into enterocyte-like cells, synthesize intestinal enzymes, and form domes. Caco-2 cells also undergo autophagy in the course of differentiation. The criteria to establish the induction of autophagy in cells are already well established. Here, we describe the protocol for the spontaneous differentiation of Caco-2 cells and the detection of autophagy using Western blot, flow cytometry, and immunofluorescence.

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Acknowledgements

This work was funded from TÜBİTAK Project no. 114S937 to S.B. Aslı Sade Memişoğlu, Orkun Cevheroglu, and members of the Banerjee lab are acknowledged for useful discussions. Cagdas Son and Mayda Gursel are acknowledged for sharing resources.

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Authors and Affiliations

  1. Department of Biological Sciences, Middle East Technical University, Ankara, Turkey

    Sinem Tunçer & Sreeparna Banerjee

Authors
  1. Sinem Tunçer
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  2. Sreeparna Banerjee
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Correspondence to Sreeparna Banerjee .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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Tunçer, S., Banerjee, S. (2017). Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells. In: Turksen, K. (eds) Autophagy in Differentiation and Tissue Maintenance. Methods in Molecular Biology, vol 1854. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_66

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  • DOI: https://doi.org/10.1007/7651_2017_66

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8747-4

  • Online ISBN: 978-1-4939-8748-1

  • eBook Packages: Springer Protocols

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