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Decellularization of Liver and Organogenesis in Rats

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Decellularized Scaffolds and Organogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1577))

Abstract

Recently, organ construction has been attempted using decellularized organs. In this study, we used decellularized rat liver to construct liver tissue by recellularization. The right lobe of the rat liver was decellularized with 4% Triton X-100 solution, recellularized with 107 rat hepatocytes, and albumin synthesis in the recellularized right lobe was observed. Therefore, we introduce a method of decellularizing rat liver, which retains its fine vascular structure after removal of all the cells, perform organogenesis using the decellularized liver, and evaluate the structural and functional properties of the products.

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Authors and Affiliations

  1. Department of Chemical Engineering, Faculty of Engineering, Kyushu University, Fukuoka, Japan

    Nana Shirakigawa & Hiroyuki Ijima

Authors
  1. Nana Shirakigawa
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  2. Hiroyuki Ijima
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Corresponding author

Correspondence to Hiroyuki Ijima .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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© 2017 Springer Science+Business Media New York

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Shirakigawa, N., Ijima, H. (2017). Decellularization of Liver and Organogenesis in Rats. In: Turksen, K. (eds) Decellularized Scaffolds and Organogenesis. Methods in Molecular Biology, vol 1577. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_63

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  • DOI: https://doi.org/10.1007/7651_2017_63

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7655-3

  • Online ISBN: 978-1-4939-7656-0

  • eBook Packages: Springer Protocols

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