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Preparation of Cell-Derived Decellularized Matrices Mimicking Native ECM During the Osteogenesis and Adipogenesis of Mesenchymal Stem Cells

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Decellularized Scaffolds and Organogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1577))

Abstract

The extracellular matrix (ECM) is an important extracellular microenvironmental factor that regulates stem cell differentiation. The ECM is remodeled according to stem cell differentiation progression to precisely regulate the differentiation. Thus, it is expected that the matrices mimicking native ECM surrounding differentiating cells at each differentiation stage provide a favorable microenvironment to promote stem cell differentiation. However, it is difficult to prepare matrices mimicking native ECM using chemical methods because the ECM has a complicated composition. The decellularization technique is useful to prepare such matrices. In this chapter, we described the protocol to prepare matrices mimicking native ECM surrounding cells that are differentiating from mesenchymal stem cells to either osteoblasts or adipocytes via stem cell differentiation culture and a detergent- and nuclease-based decellularization technique.

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Acknowledgments

This work was supported by a Grant-in-Aid for Young Scientists (A) (26702016) and a Grant-in-Aid for Challenging Exploratory Research (16K15209), funded by MEXT, Japan.

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Authors and Affiliations

  1. Frontier Center for Organic Materials, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata, 992-8510, Japan

    Takashi Hoshiba

  2. Innovative Flex Course for Frontier Organic Materials Systems, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata, 992-8510, Japan

    Takashi Hoshiba

  3. International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan

    Takashi Hoshiba, Naoki Kawazoe & Guoping Chen

Authors
  1. Takashi Hoshiba
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  2. Naoki Kawazoe
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  3. Guoping Chen
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Corresponding author

Correspondence to Takashi Hoshiba .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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Hoshiba, T., Kawazoe, N., Chen, G. (2017). Preparation of Cell-Derived Decellularized Matrices Mimicking Native ECM During the Osteogenesis and Adipogenesis of Mesenchymal Stem Cells. In: Turksen, K. (eds) Decellularized Scaffolds and Organogenesis. Methods in Molecular Biology, vol 1577. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_62

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  • DOI: https://doi.org/10.1007/7651_2017_62

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7655-3

  • Online ISBN: 978-1-4939-7656-0

  • eBook Packages: Springer Protocols

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