Skip to main content
SpringerLink
Log in

Wharton’s Jelly Matrix Decellularization for Tissue Engineering Applications

  • Protocol
  • First Online:
Book cover Decellularized Scaffolds and Organogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1577))

Abstract

Scaffolds, both natural and synthetic, used in tissue engineering provide mechanical support to cells. Tissue decellularization has been used to provide natural extracellular matrix scaffolds for tissue engineering purposes. In this chapter we focus on describing the methodology used to decellularize Wharton’s jelly matrix, the mucous connective tissue that surrounds umbilical cord vessels, to obtain decellularized Wharton’s jelly matrix (DWJM); an extracellular matrix that can be used for tissue engineering purposes. We also, briefly, describe our experience with processing DWJM for cell seeding and recellularization.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Institutional subscriptions

References

  1. Jadalannagari S, Converse G, McFall C, Buse E, Filla M, Villar MT, Artigues A, Mellot AJ, Wang J, Detamore MS, Hopkins RA, Aljitawi OS (2017) Decellularized Wharton’s Jelly from human umbilical cord as a novel 3D scaffolding material for tissue engineering applications. PLoS One 12(2):e0172098

    Article  Google Scholar 

  2. Zippel N, Schulze M, Tobiasch E (2009) Biomaterials and Mesenchymal stem cells for regenerative medicine. Recent Pat Biotechnol. doi: BIOT-ABSTRACT 08 [pii]

    Google Scholar 

  3. Hellstrom M, El-Akouri RR, Sihlbom C, Olsson BM, Lengqvist J, Backdahl H, Johansson BR, Olausson M, Sumitran-Holgersson S, Brannstrom M (2014) Towards the development of a bioengineered uterus: comparison of different protocols for rat uterus decellularization. Acta Biomater 10(12):5034–5042

    Article  CAS  Google Scholar 

  4. Costa F, Dohmen P, Vieira E, Lopes SV, Colatusso C, Pereira EWL, Matsuda CN, Cauduro S (2007) Ross operation with decellularized pulmonary allografts: medium-term results. Rev Bras Cir Cardiovasc 22(4):454–462

    Article  Google Scholar 

  5. Kiyotake EA, Beck EC, Detamore MS (2016) Cartilage extracellular matrix as a biomaterial for cartilage regeneration. Ann N Y Acad Sci 1383(1):139–159

    Article  CAS  Google Scholar 

  6. Law JX, Liau LL, Aminuddin BS, Ruszymah BHI (2016) Tissue-engineered trachea: a review. Int J Pediatr Otorhinolaryngol 91:55–63

    Article  Google Scholar 

  7. Franc S, Rousseau JC, Garrone R, van der Rest M, Moradi-Ameli M (1998) Microfibrillar composition of umbilical cord matrix: characterization of fibrillin, collagen VI and intact collagen V. Placenta 19(1):95–104

    Article  CAS  Google Scholar 

  8. Ferguson VL, Dodson RB (2009) Bioengineering aspects of the umbilical cord. Eur J Obstet Gynecol Reprod Biol 144(Suppl 1):S108–S113. doi: S0301-2115(09)00133-X [pii]10.1016/j.ejogrb.2009.02.024

    Article  Google Scholar 

  9. Grayson WL, Zhao F, Izadpanah R, Bunnell B, Ma T (2006) Effects of hypoxia on human mesenchymal stem cell expansion and plasticity in 3D constructs. J Cell Physiol 207(2):331–339. doi:10.1002/jcp.20571

    Article  CAS  PubMed  Google Scholar 

  10. Bastow ER, Byers S, Golub SB, Clarkin CE, Pitsillides AA, Fosang AJ (2008) Hyaluronan synthesis and degradation in cartilage and bone. Cell Mol Life Sci 65(3):395–413. doi:10.1007/s00018-007-7360-z

    Article  CAS  PubMed  Google Scholar 

  11. Ishige I, Nagamura-Inoue T, Honda MJ, Harnprasopwat R, Kido M, Sugimoto M, Nakauchi H, Tojo A (2009) Comparison of mesenchymal stem cells derived from arterial, venous, and Wharton’s Jelly explants of human umbilical cord. Int J Hematol 90(2):261–269. doi:10.1007/s12185-009-0377-3

    Article  PubMed  Google Scholar 

  12. Sobolewski K, Malkowski A, Bankowski E, Jaworski S (2005) Wharton’s Jelly as a reservoir of peptide growth factors. Placenta 26(10):747–752. doi:S0143-4004(04)00260-7 [pii] 10.1016/j.placenta.2004.10.008

    Article  CAS  Google Scholar 

  13. Taipale J, Keski-Oja J (1997) Growth factors in the extracellular matrix. FASEB J 11(1):51–59

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Children’s Mercy-Kansas City, Kansas City, MO, 64108, USA

    Gabriel L. Converse, Eric E. Buse & Richard A. Hopkins

  2. University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY, 14642, USA

    Dandan Li & Omar S. Aljitawi

Authors
  1. Gabriel L. Converse
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dandan Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Eric E. Buse
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Richard A. Hopkins
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Omar S. Aljitawi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Omar S. Aljitawi .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Science+Business Media New York

About this protocol

Cite this protocol

Converse, G.L., Li, D., Buse, E.E., Hopkins, R.A., Aljitawi, O.S. (2017). Wharton’s Jelly Matrix Decellularization for Tissue Engineering Applications. In: Turksen, K. (eds) Decellularized Scaffolds and Organogenesis. Methods in Molecular Biology, vol 1577. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_61

Download citation

  • DOI: https://doi.org/10.1007/7651_2017_61

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7655-3

  • Online ISBN: 978-1-4939-7656-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation