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A Cleanup Method for Mass Spectrometric Analysis of Sphingosine- and Ceramide-1-Phosphate in Blood and Solid Tissue Using a Phosphate Capture Molecule

  • Jun-ichi Morishige
  • Ryouhei Yamashita
  • Tamotsu Tanaka
  • Kiyoshi Satouchi
Protocol
Part of the Methods in Molecular Biology book series

Abstract

Cleanup technology and mass spectrometric determination of sphingosine-1-phosphate (S1P) using a phosphate capture molecule are shown. The protocol is rapid, requires neither thin-layer chromatography nor liquid chromatography, and is applicable to both blood and solid tissue samples. The mass spectrometric method is also applicable to ceramide-1-phosphate.

Keywords:

Ceramide-1-phosphate Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Phos-tag Sphingosine-1-phosphate 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Jun-ichi Morishige
    • 1
  • Ryouhei Yamashita
    • 2
  • Tamotsu Tanaka
    • 2
  • Kiyoshi Satouchi
    • 3
  1. 1.Department of Cellular and Molecular Function AnalysisKanazawa University Graduate School of Medical ScienceKanazawaJapan
  2. 2.Institute of Biomedical SciencesTokushima University Graduate SchoolTokushimaJapan
  3. 3.Department of Applied Biological ScienceFukuyama UniversityFukuyamaJapan

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