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Decellularization and Recellularization of Cartilage

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Decellularized Scaffolds and Organogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1577))

Abstract

Decellularization of cartilage enables the use of cartilage allografts or xenografts as natural scaffolds for repair and regeneration of injured cartilage. The preservation of the extracellular matrix ultrastructure of the graft makes this a promising tool for cartilage tissue engineering. We have optimized the decellularization protocol by enzymatically digesting proteoglycans while preserving the native collagen architecture. Here we describe our methods for cartilage decellularization and cell labeling for the tracking of infiltration for recellularization in detail.

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References

  1. Sirlin CB, Brossmann J, Boutin RD, Pathria MN, Convery FR, Bugbee W, Deutsch R, Lebeck LK, Resnick D (2001) Shell osteochondral allografts of the knee: comparison of mr imaging findings and immunologic responses. Radiology 219(1):35–43

    Article  CAS  PubMed  Google Scholar 

  2. Pallante AL, Görtz S, Chen AC, Healey RM, Chase DC, Ball ST, Amiel D, Sah RL, Bugbee WD (2012) Treatment of articular cartilage defects in the goat with frozen versus fresh osteochondral allografts: effects on cartilage stiffness, zonal composition, and structure at six months. J Bone Joint Surg Am 94(21):1984–1995. doi:10.2106/JBJS.K.00439

    Article  PubMed  PubMed Central  Google Scholar 

  3. Londono R, Dziki JL, Haljasmaa E, Turner N, Leifer C, Badylak SF (2017) The effect of cell debris within biologic scaffolds upon the macrophage response. J Biomed Mater Res A. doi:10.1002/jbm.a.36055

    Article  CAS  PubMed  Google Scholar 

  4. Camarero-Espinosa S, Rothen-Rutishauser B, Foster EJ, Weder C (2016) Articular cartilage: from formation to tissue engineering. Biomater Sci 4(5):734–767. doi:10.1039/c6bm00068a

    Article  CAS  PubMed  Google Scholar 

  5. Sutherland AJ, Converse GL, Hopkins RA, Detamore MS (2015) The bioactivity of cartilage extracellular matrix in articular cartilage regeneration. Adv Healthc Mater 4(1):29–39. doi:10.1002/adhm.201400165

    Article  CAS  PubMed  Google Scholar 

  6. Cheng CW, Solorio LD, Alsberg E (2014) Decellularized tissue and cell-derived extracellular matrices as scaffolds for orthopaedic tissue engineering. Biotechnol Adv 32(2):462–484. doi:10.1016/j.biotechadv.2013.12.012

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Fermor HL, Russell SL, Williams S, Fisher J, Ingham E (2015) Development and characterisation of a decellularised bovine osteochondral biomaterial for cartilage repair. J Mater Sci Mater Med 26(5):186. doi:10.1007/s10856-015-5517-0

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Elder BD, Kim DH, Athanasiou KA (2010) Developing an articular cartilage decellularization process toward facet joint cartilage replacement. Neurosurgery 66(4):722–727.; discussion 727. doi:10.1227/01.NEU.0000367616.49291.9F

    Article  PubMed  Google Scholar 

  9. Luo L, Chu JY, Eswaramoorthy R, Mulhall KJ, Kelly DJ (2016) Engineering tissues that mimic the zonal nature of articular cartilage using decellularized cartilage explants seeded with adult stem cells. ACS Biomater Sci Eng. doi:10.1021/acsbiomaterials.6b00020

    Article  Google Scholar 

  10. Utomo L, Pleumeekers MM, Nimeskern L, Nürnberger S, Stok KS, Hildner F et al (2015) Preparation and characterization of a decellularized cartilage scaffold for ear cartilage reconstruction. Biomed Mater 10(1):015010. doi:10.1088/1748-6041/10/1/01501

    Article  PubMed  Google Scholar 

  11. Schwarz S, Elsaesser AF, Koerber L, Goldberg-Bockhorn E, Seitz AM, Bermueller C et al (2015) Processed xenogenic cartilage as innovative biomatrix for cartilage tissue engineering: effects on chondrocyte differentiation and function. J Tissue Eng Regen Med 9(12):E239–E251. doi:10.1002/term.1650

    Article  CAS  PubMed  Google Scholar 

  12. Bautista CA, Park HJ, Mazur CM, Aaron RK, Bilgen B (2016) Effects of chondroitinase ABC-mediated proteoglycan digestion on decellularization and recellularization of articular cartilage. PLoS One 11(7):e0158976. doi:10.1371/journal.pone.0158976

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

We would like to acknowledge the assistance of Courtney M. Mazur in protocol development. This work was supported by Career Development Award # IK2 RX000760 from the United States (U.S.) Department of Veterans Affairs Rehabilitation Research and Development Service.

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Authors and Affiliations

  1. University of California, San Diego, CA, USA

    Catherine A. Bautista

  2. Providence VA Medical Center, Providence, RI, USA

    Bahar Bilgen

  3. Department of Orthopaedics, The Warren Alpert Medical School of Brown University, Rhode Island Hospital, 1 Hoppin Street, Providence, RI, USA

    Bahar Bilgen

Authors
  1. Catherine A. Bautista
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  2. Bahar Bilgen
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Corresponding author

Correspondence to Bahar Bilgen .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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© 2017 Springer Science+Business Media New York

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Bautista, C.A., Bilgen, B. (2017). Decellularization and Recellularization of Cartilage. In: Turksen, K. (eds) Decellularized Scaffolds and Organogenesis. Methods in Molecular Biology, vol 1577. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_59

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  • DOI: https://doi.org/10.1007/7651_2017_59

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7655-3

  • Online ISBN: 978-1-4939-7656-0

  • eBook Packages: Springer Protocols

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