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Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells

Protocol
Part of the Methods in Molecular Biology book series

Abstract

Organoid systems take advantage of the self-organizing capabilities of cells to create diverse multi-cellular tissue surrogates that constitute a powerful novel class of biological models. Clearly, the formation of a testicular organoid (TO) in which human spermatogenesis can proceed from a single-cell suspension would exert a tremendous impact on research and development, clinical treatment of infertility, and screening of potential drugs and toxic agents. Recently, we showed that primary adult and pubertal human testicular cells auto-assembled in TOs either with or without the support of a natural testis scaffold. These mini-tissues harboured both the spermatogonial stem cells and their important niche cells, which retained certain specific functions during long-term culture. As such, human TOs might advance the development of a system allowing human in vitro spermatogenesis. Here we describe the methodology to make scaffold-based and scaffold-free TOs.

Keywords

Testis Organoid Primary cells Extracellular matrix Scaffold In vitro spermatogenesis 

Notes

Acknowledgements

Financial support was obtained from the Agency for Innovation by Science and Technology (IWT) and the Vrije Universiteit Brussel, Y.B. is a postdoctoral fellow of the Scientific Research Foundation Flanders (FWO).

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Biology of the Testis, Research Laboratory for Reproduction, Genetics and Regenerative MedicineVrije Universiteit Brussel (VUB)BrusselsBelgium

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