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Alveolar Rhabdomyosarcoma Decellularization
In cancer research, it is an urgent need in the obtainment of a simple and reproducible model that mimics in all the complexity the pathological microenvironment. Specifically, the will to improve the overall survival of young patients affected by rhabdomyosarcoma compels researchers to develop new models resembling the multifaceted environment of the pathology to deeply study the disease under novel and different aspects. To this end, we developed a decellularization protocol for alveolar rhabdomyosarcoma (ARMS) able to maintain the three-dimensional structure. The attained extracellular matrix (ECM) can be used as 3D in vitro model suitable to both recapitulate the in vivo cancer microenvironment, and also for drug testing. Here, we first describe a detergent-enzymatic method and then we analyze the decellularization efficiency and the scaffold proteins.
Keywords:Decellularization Extracellular matrix Rhabdomyosarcoma 3D in vitro model
Saggioro M and D’Agostino S are supported by Istituto di Ricerca Pediatrica Città della Speranza. Pozzobon M is supported by University of Padova, Grant number GRIC15AIPF, Assegno di ricerca Senior. Pozzobon M is a coinventor of the Italian Patent N. 0001422436 entitled “Matrice acellulare per ricostruzione in vivo di muscolo scheletrico.”
- 2.Hettmer S, Li Z, Billin AN et al (2014) Rhabdomyosarcoma: current challenges and their implications for developing therapies. Cold Spring Harb Perspect Med. doi: 10.1101/cshperspect.a025650