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Alveolar Rhabdomyosarcoma Decellularization

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Decellularized Scaffolds and Organogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1577))

Abstract

In cancer research, it is an urgent need in the obtainment of a simple and reproducible model that mimics in all the complexity the pathological microenvironment. Specifically, the will to improve the overall survival of young patients affected by rhabdomyosarcoma compels researchers to develop new models resembling the multifaceted environment of the pathology to deeply study the disease under novel and different aspects. To this end, we developed a decellularization protocol for alveolar rhabdomyosarcoma (ARMS) able to maintain the three-dimensional structure. The attained extracellular matrix (ECM) can be used as 3D in vitro model suitable to both recapitulate the in vivo cancer microenvironment, and also for drug testing. Here, we first describe a detergent-enzymatic method and then we analyze the decellularization efficiency and the scaffold proteins.

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Acknowledgments

Saggioro M and D’Agostino S are supported by Istituto di Ricerca Pediatrica Città della Speranza. Pozzobon M is supported by University of Padova, Grant number GRIC15AIPF, Assegno di ricerca Senior. Pozzobon M is a coinventor of the Italian Patent N. 0001422436 entitled “Matrice acellulare per ricostruzione in vivo di muscolo scheletrico.”

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Authors and Affiliations

  1. Department of Women’s and Children’s Health, University of Padova, Padova, Italy

    Michela Pozzobon, Mattia Saggioro, Gianni Bisogno, Maurizio Muraca & Piergiorgio Gamba

  2. Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Padova, Italy

    Michela Pozzobon, Mattia Saggioro & Stefania D’Agostino

  3. Stem Cells and Regenerative Medicine Lab, Istituto di Ricerca Pediatrica Città della Speranza, Corso Stati Uniti 4, 35127, Padova, Italy

    Michela Pozzobon

Authors
  1. Michela Pozzobon
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  2. Mattia Saggioro
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  3. Stefania D’Agostino
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  4. Gianni Bisogno
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  5. Maurizio Muraca
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  6. Piergiorgio Gamba
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Corresponding author

Correspondence to Michela Pozzobon .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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© 2017 Springer Science+Business Media New York

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Pozzobon, M., Saggioro, M., D’Agostino, S., Bisogno, G., Muraca, M., Gamba, P. (2017). Alveolar Rhabdomyosarcoma Decellularization. In: Turksen, K. (eds) Decellularized Scaffolds and Organogenesis. Methods in Molecular Biology, vol 1577. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_45

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  • DOI: https://doi.org/10.1007/7651_2017_45

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7655-3

  • Online ISBN: 978-1-4939-7656-0

  • eBook Packages: Springer Protocols

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