Protocol

Part of the series Methods in Molecular Biology pp 1-9

Date:

Alveolar Rhabdomyosarcoma Decellularization

  • Michela PozzobonAffiliated withDepartment of Women’s and Children’s Health, University of PadovaFondazione Istituto di Ricerca Pediatrica Città della SperanzaStem Cells and Regenerative Medicine Lab, Istituto di Ricerca Pediatrica Città della Speranza Email author 
  • , Mattia SaggioroAffiliated withDepartment of Women’s and Children’s Health, University of PadovaFondazione Istituto di Ricerca Pediatrica Città della Speranza
  • , Stefania D’AgostinoAffiliated withFondazione Istituto di Ricerca Pediatrica Città della Speranza
  • , Gianni BisognoAffiliated withDepartment of Women’s and Children’s Health, University of Padova
  • , Maurizio MuracaAffiliated withDepartment of Women’s and Children’s Health, University of Padova
  • , Piergiorgio GambaAffiliated withDepartment of Women’s and Children’s Health, University of Padova

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Abstract

In cancer research, it is an urgent need in the obtainment of a simple and reproducible model that mimics in all the complexity the pathological microenvironment. Specifically, the will to improve the overall survival of young patients affected by rhabdomyosarcoma compels researchers to develop new models resembling the multifaceted environment of the pathology to deeply study the disease under novel and different aspects. To this end, we developed a decellularization protocol for alveolar rhabdomyosarcoma (ARMS) able to maintain the three-dimensional structure. The attained extracellular matrix (ECM) can be used as 3D in vitro model suitable to both recapitulate the in vivo cancer microenvironment, and also for drug testing. Here, we first describe a detergent-enzymatic method and then we analyze the decellularization efficiency and the scaffold proteins.

Keywords:

Decellularization Extracellular matrix Rhabdomyosarcoma 3D in vitro model