pp 1-12 | Cite as
Monitoring of Iron Depletion-Induced Mitophagy in Pathogenic Yeast
Mitophagy, which is the degradation of mitochondria via selective autophagic machinery, is thought to be involved in regulating the mass and function of mitochondria. Methods for detection of mitophagy have been reported for several fungal cells including some budding yeast, methylotrophic yeast, and filamentous fungi. Mitophagy in Saccharomyces cerevisiae is activated under nitrogen-poor conditions; however, the regulatory mechanism of mitophagy in most fungi has not been elucidated. Here we describe methods to monitor mitophagy in the pathogenic yeast Candida glabrata under iron-depleted conditions but not under nitrogen starvation. This observation may provide some clues to elucidate the physiological roles of mitophagy in eukaryotes.
KeywordsAtg32 Autophagy Candida Iron Mitochondria Mitophagy Pathogenicity Yeast
Green fluorescent protein
Phenylmethane sulfonyl fluoride
Reactive oxygen species
Synthetic glucose medium
Tris-buffered saline containing Tween 20
Yeast extract peptone dextrose
We thank Koji and Noriko Okamoto (Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan) for providing yeast strains and plasmids. This research was supported by JSPS KAKENHI Grant Numbers 26790428 (KT), 24590540 (HN), and 26860296 (MN). This work was partly supported by a grant from the Ministry of Health, Labour and Welfare of Japan (H26-shinkoujitsuyouka-ippan-010). The authors would like to thank Enago (www.enago.jp) for the English language review.
- 2.Kurihara Y, Kanki T, Aoki Y, Hirota Y, Saigusa T, Uchiumi T, Kang D (2012) Mitophagy plays an essential role in reducing mitochondrial production of reactive oxygen species and mutation of mitochondrial DNA by maintaining mitochondrial quantity and quality in yeast. J Biol Chem 287:3265–3272CrossRefPubMedGoogle Scholar