pp 1-18

Part of the Methods in Molecular Biology book series

3D-Printing Composite Polycaprolactone-Decellularized Bone Matrix Scaffolds for Bone Tissue Engineering Applications

  • Alexandra N. Rindone
  • Ethan Nyberg
  • Warren L. Grayson
Protocol

Abstract

Millions of patients worldwide require bone grafts for treatment of large, critically sized bone defects from conditions such as trauma, cancer, and congenital defects. Tissue engineered (TE) bone grafts have the potential to provide a more effective treatment than current bone grafts since they would restore fully functional bone tissue in large defects. Most bone TE approaches involve a combination of stem cells with porous, biodegradable scaffolds that provide mechanical support and degrade gradually as bone tissue is regenerated by stem cells. 3D-printing is a key technique in bone TE that can be used to fabricate functionalized scaffolds with patient-specific geometry. Using 3D-printing, composite polycaprolactone (PCL) and decellularized bone matrix (DCB) scaffolds can be produced to have the desired mechanical properties, geometry, and osteoinductivity needed for a TE bone graft. This book chapter will describe the protocols for fabricating and characterizing 3D-printed PCL:DCB scaffolds. Moreover, procedures for culturing adipose-derived stem cells (ASCs) in these scaffolds in vitro will be described to demonstrate the osteoinductivity of the scaffolds.

Keywords

Bone tissue engineering 3D-printing Decellularized bone matrix Polycaprolactone Adipose-derived stem cells 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Alexandra N. Rindone
    • 1
    • 2
  • Ethan Nyberg
    • 1
    • 2
  • Warren L. Grayson
    • 1
    • 2
    • 3
    • 4
    • 5
  1. 1.Translational TE CenterJohns Hopkins University School of MedicineBaltimoreUSA
  2. 2.Department of Biomedical EngineeringJohns Hopkins University School of MedicineBaltimoreUSA
  3. 3.Department of Materials Science and EngineeringJohns Hopkins UniversityBaltimoreUSA
  4. 4.Institute for NanoBioTechnologyJohns Hopkins UniversityBaltimoreUSA
  5. 5.Johns Hopkins University, Translational Tissue Engineering CenterBaltimoreUSA

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