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Decellularization Methods for Scaffold Fabrication

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Decellularized Scaffolds and Organogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1577))

  • The original version of this chapter was revised. An erratum to this chapter can be found at DOI 10.1007/7651_2017_110

Abstract

Decellularization is the process of removal of native cells from tissue, leaving behind a three-dimensional (3D) ultrastructure of extracellular matrix (ECM) proteins while preserving the bioactivity and mechanics of the tissue. It offers a unique top-down approach for fabricating ECM based natural scaffold for tissue engineering application. Herein, this chapter presents the fabrication of decellularized scaffold employing different methods: whole organ perfusion, immersion and agitation, pressure gradient, and supercritical fluid. The decellularized scaffold aims to exploit the nature-designed 3D architecture, a successful platform technology, for creating scaffolding materials for tissue engineering and regenerative medicine.

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Change history

  • 14 January 2018

    The publisher regrets that an author was not mentioned in the chapter by mistake. The details of the author are provided below:

    Archna Dhasmana – Department of Polymer and Process Engineering, Indian Institute of Technology, Roorkee, India

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Acknowledgements

The authors are thankful to the Council of Scientific and Industrial Research (Grant No. 27(0222)/10/EM R-II dated 31.05.10), India, for the financial support of this work.

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Authors and Affiliations

  1. Department of Polymer and Process Engineering, Indian Institute of Technology, Roorkee, 247001, India

    Sweta K. Gupta, Narayan C. Mishra & Archna Dhasmana

  2. Department of Chemical Engineering, University of Rhode Island, Kingston, RI, 02881, USA

    Sweta K. Gupta

Authors
  1. Sweta K. Gupta
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  2. Narayan C. Mishra
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  3. Archna Dhasmana
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Corresponding author

Correspondence to Narayan C. Mishra .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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Gupta, S.K., Mishra, N.C., Dhasmana, A. (2017). Decellularization Methods for Scaffold Fabrication. In: Turksen, K. (eds) Decellularized Scaffolds and Organogenesis. Methods in Molecular Biology, vol 1577. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_34

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  • DOI: https://doi.org/10.1007/7651_2017_34

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7655-3

  • Online ISBN: 978-1-4939-7656-0

  • eBook Packages: Springer Protocols

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