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Part of the series Methods in Molecular Biology pp 1-10

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Decellularization Methods for Scaffold Fabrication

  • Sweta K. GuptaAffiliated withDepartment of Polymer and Process Engineering, Indian Institute of TechnologyDepartment of Chemical Engineering, University of Rhode Island
  • , Narayan C. MishraAffiliated withDepartment of Polymer and Process Engineering, Indian Institute of Technology Email author 

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Abstract

Decellularization is the process of removal of native cells from tissue, leaving behind a three-dimensional (3D) ultrastructure of extracellular matrix (ECM) proteins while preserving the bioactivity and mechanics of the tissue. It offers a unique top-down approach for fabricating ECM based natural scaffold for tissue engineering application. Herein, this chapter presents the fabrication of decellularized scaffold employing different methods: whole organ perfusion, immersion and agitation, pressure gradient, and supercritical fluid. The decellularized scaffold aims to exploit the nature-designed 3D architecture, a successful platform technology, for creating scaffolding materials for tissue engineering and regenerative medicine.

Keywords

Decellularization Scaffold Cells Extracellular matrix Tissue engineering