pp 1-10 | Cite as
Decellularization of Bovine Small Intestinal Submucosa
Decellularization technology promises to overcome some of the significant limitations in the regenerative medicine field by providing functional biocompatible grafts. The technique involves removal of the cells from the biological tissues or organs for further use in tissue engineering and clinical interventions. There are significant differences between decellularization protocols due to the intrinsic properties of different tissue types and purpose of use. This multistep, chemical-solution-based protocol is optimized for the preparation of decellularized bovine small intestinal submucosa (SIS).
Keywords:Decellularization Bovine Small intestinal submucosa (SIS) Xenogenic biomaterials Extracellular matrix (ECM) Regenerative medicine Bioactive materials
Y.M.E. is the founder of Biovalda, Inc. (Ankara, Turkey) and has intellectual properties related to decellularized tissues.
- 2.Parmaksiz M, Dogan A, Odabas S, Elcin AE, Elcin YM (2016) Clinical applications of decellularized extracellular matrices for tissue engineering and regenerative medicine. Topical review. Biomed Mater 11(2):022003Google Scholar
- 7.Parmaksiz M, Elcin AE, Elcin YM (2015) Decellularization of bovine small intestinal submucosa and its use for the healing of a critical-sized full-thickness skin defect, alone and in combination with stem cells, in a small rodent model. J Tissue Eng Regen Med. doi: 10.1002/term.2071 (Epub ahead of print)PubMedGoogle Scholar
- 16.Kawecki M, Łabuś W, Klama-Baryla A, Kitala D, Kraut M, Glik J, Misiuga M, Nowak M, Bielecki T, Kasperczyk A (2017) A review of decellurization methods caused by an urgent need for quality control of cell-free extracellular matrix scaffolds and their role in regenerative medicine. J Biomed Mater Res B Appl Biomater. doi: 10.1002/jbm.b.33865 (Epub ahead of print)Google Scholar
- 17.Brown BN, Freund JM, Han L, Rubin JP, Reing JE, Jeffries EM, Wolf MT, Tottey S, Barnes CA, Ratner BD, Badylak SF (2011) Comparison of three methods for the derivation of a biologic scaffold composed of adipose tissue extracellular matrix. Tissue Eng Part C Methods 17:411–421CrossRefPubMedPubMedCentralGoogle Scholar
- 20.Xu H, Xu B, Yang Q, Li X, Ma X, Xia Q, Zhang Y, Zhang C, Wu Y, Zhang Y (2014) Comparison of decellularization protocols for preparing a decellularized porcine annulus fibrosus scaffold. PLoS One 9:1–13Google Scholar