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Decellularization of Intact Lung Tissue Through Vasculature and Airways Using Negative and Positive Pressure

Protocol
Part of the Methods in Molecular Biology book series

Abstract

Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.

Keywords

Decellularization Lung Negative pressure bioreactor Organ scaffold 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Steven Skolasinski
    • 1
  • Angela Panoskaltsis-Mortari
    • 2
  1. 1.Department of Medicine, Division of Pulmonary, Allergy, Critical Care, and sleep350 Variety Heart and Research Center, University of MinnesotaMinneapolisUSA
  2. 2.Department of Pediatrics, Blood and Marrow Transplant ProgramUniversity of MinnesotaMinneapolisUSA

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