Decellularization of Intact Lung Tissue Through Vasculature and Airways Using Negative and Positive Pressure
Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.
KeywordsDecellularization Lung Negative pressure bioreactor Organ scaffold
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