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Decellularization of Intact Lung Tissue Through Vasculature and Airways Using Negative and Positive Pressure

Part of the Methods in Molecular Biology book series


Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.


Decellularization Lung Negative pressure bioreactor Organ scaffold 


  1. 1.
    Price AP, England KA, Matson AM et al (2010) Development of a decellularized lung bioreactor system for bioengineering the lung: the matrix reloaded. Tissue Eng Part A 16:2581–2591. doi: 10.1089/ten.TEA.2009.0659 CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Crabbé A, Liu Y, Sarker SF et al (2015) Recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture. PLoS One 10:1–17. doi: 10.1371/journal.pone.0126846 CrossRefGoogle Scholar
  3. 3.
    Nichols JE, La Francesca S, Vega SP et al (2016) Giving new life to old lungs: methods to produce and assess whole human paediatric bioengineered lungs. J Tissue Eng Regen Med n/a-n/a. Doi:  10.1002/term.2113
  4. 4.
    Franks TJ, Colby TV, Travis WD et al (2008) Resident cellular components of the human lung: current knowledge and goals for research on cell phenotyping and function. Proc Am Thorac Soc 5:763–766. doi: 10.1513/pats.200803-025HR CrossRefPubMedGoogle Scholar
  5. 5.
    Stone KC, Mercer RR, Freeman BA et al (1992) Distribution of lung cell numbers and volumes between alveolar and nonalveolar tissue. Am Rev Respir Dis 146:454–456. doi: 10.1164/ajrccm/146.2.454 CrossRefPubMedGoogle Scholar
  6. 6.
    Tsuchiya T, Mendez J, Calle EA et al (2016) Ventilation-based decellularization system of the lung. Biores Open Access 5:118–126. doi: 10.1089/biores.2016.0012 CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Raredon MSB, Rocco KA, Gheorghe CP et al (2016) Biomimetic culture reactor for whole-lung engineering. Biores Open Access 5:72–83. doi: 10.1089/biores.2016.0006 CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    He M, Callanan A (2013) Comparison of methods for whole-organ decellularization in tissue engineering of bioartificial organs. Tissue Eng Part B Rev 19:194–208. doi: 10.1089/ten.teb.2012.0340 CrossRefPubMedGoogle Scholar
  9. 9.
    Price AP, Godin LM, Domek A et al (2015) Automated decellularization of intact, human-sized lungs for tissue engineering. Tissue Eng Part C Methods 21:94–103. doi: 10.1089/ten.TEC.2013.0756 CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Steven Skolasinski
    • 1
  • Angela Panoskaltsis-Mortari
    • 2
  1. 1.Department of Medicine, Division of Pulmonary, Allergy, Critical Care, and sleep350 Variety Heart and Research Center, University of MinnesotaMinneapolisUSA
  2. 2.Department of Pediatrics, Blood and Marrow Transplant ProgramUniversity of MinnesotaMinneapolisUSA

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