Protocol

Part of the series Methods in Molecular Biology pp 1-15

Date:

Decellularized Iliotibial Band Recolonized with Allogenic Homotopic Fibroblasts or Bone Marrow-Derived Mesenchymal Stromal Cells

  • Clemens GögeleAffiliated withInstitute of Anatomy, Paracelsus Medical University, Salzburg and Nuremberg 
  • , Silke SchwarzAffiliated withInstitute of Anatomy, Paracelsus Medical University, Salzburg and Nuremberg 
  • , Benjamin OndruschkaAffiliated withInstitute of Legal Medicine, University of Leipzig
  • , Niels HammerAffiliated withDepartment of Anatomy, Otago School of Biomedical Sciences, University of Otago
  • , Gundula Schulze-TanzilAffiliated withInstitute of Anatomy, Paracelsus Medical University, Salzburg and Nuremberg Email author 

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Abstract

Decellularized scaffolds present promising biomimetic approaches in various fields of tissue engineering. Different tissues have been selected for decellularization, among them extracellular matrix (ECM)-rich tissues such as tendons, ligaments and cartilage. The dense ECM of ligaments is particularly challenging to achieve a completely non-immunogenic ECM void of any cells. Here, the methods for decellularization adapted to ligamentous tissue of the iliotibial band (ITB) are presented along with cell isolation and several recolonization techniques using allogenic ITB-derived fibroblasts or mesenchymal stromal cells (MSCs).

Keywords:

Acellularization Decellularization Explant culture Iliotibial band Ligamentocytes Mesenchymal stromal cells Recellularization