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Decellularized Iliotibial Band Recolonized with Allogenic Homotopic Fibroblasts or Bone Marrow-Derived Mesenchymal Stromal Cells
- First Online:
Abstract
Decellularized scaffolds present promising biomimetic approaches in various fields of tissue engineering. Different tissues have been selected for decellularization, among them extracellular matrix (ECM)-rich tissues such as tendons, ligaments and cartilage. The dense ECM of ligaments is particularly challenging to achieve a completely non-immunogenic ECM void of any cells. Here, the methods for decellularization adapted to ligamentous tissue of the iliotibial band (ITB) are presented along with cell isolation and several recolonization techniques using allogenic ITB-derived fibroblasts or mesenchymal stromal cells (MSCs).
Keywords:
Acellularization Decellularization Explant culture Iliotibial band Ligamentocytes Mesenchymal stromal cells RecellularizationAbbreviations
- AB
Alcian blue
- ACL
Anterior cruciate ligament
- dest
Aqua destillata
- BSA
Bovine serum albumin
- DAPI
4′,6-Diamidino-2-phenylindol
- DMSO
Dimethylsulfoxide
- DMEM
Dulbecco’s Minimum Essential Medium
- DP
Decellularization process
- ECM
Extracellular matrix
- EDTA
Ethylenediaminetetraacetic acid
- FBS
Foetal bovine serum
- FDA
Fluorescein diacetate
- HE
Hematoxylin and eosin
- ITB
Iliotibial band
- MSC
Mesenchymal stromal cells
- MTS
3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium
- PBS
Phosphate buffered saline
- PET
Polyethylene terephthalate
- PI
Propidium iodide
- rpm
Rounds per minute
- RPMI
Roswell Park Memorial Institute medium
- RT
Room temperature
- SDS
Sodium dodecyl sulphate
- 3D
Three-dimensional
- TBS
Tris buffered saline
- TRIS
Tris-(hydroxymethyl)-aminomethane
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