pp 1-15 | Cite as
Decellularized Iliotibial Band Recolonized with Allogenic Homotopic Fibroblasts or Bone Marrow-Derived Mesenchymal Stromal Cells
Abstract
Decellularized scaffolds present promising biomimetic approaches in various fields of tissue engineering. Different tissues have been selected for decellularization, among them extracellular matrix (ECM)-rich tissues such as tendons, ligaments and cartilage. The dense ECM of ligaments is particularly challenging to achieve a completely non-immunogenic ECM void of any cells. Here, the methods for decellularization adapted to ligamentous tissue of the iliotibial band (ITB) are presented along with cell isolation and several recolonization techniques using allogenic ITB-derived fibroblasts or mesenchymal stromal cells (MSCs).
Keywords:
Acellularization Decellularization Explant culture Iliotibial band Ligamentocytes Mesenchymal stromal cells RecellularizationAbbreviations
- AB
Alcian blue
- ACL
Anterior cruciate ligament
- dest
Aqua destillata
- BSA
Bovine serum albumin
- DAPI
4′,6-Diamidino-2-phenylindol
- DMSO
Dimethylsulfoxide
- DMEM
Dulbecco’s Minimum Essential Medium
- DP
Decellularization process
- ECM
Extracellular matrix
- EDTA
Ethylenediaminetetraacetic acid
- FBS
Foetal bovine serum
- FDA
Fluorescein diacetate
- HE
Hematoxylin and eosin
- ITB
Iliotibial band
- MSC
Mesenchymal stromal cells
- MTS
3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium
- PBS
Phosphate buffered saline
- PET
Polyethylene terephthalate
- PI
Propidium iodide
- rpm
Rounds per minute
- RPMI
Roswell Park Memorial Institute medium
- RT
Room temperature
- SDS
Sodium dodecyl sulphate
- 3D
Three-dimensional
- TBS
Tris buffered saline
- TRIS
Tris-(hydroxymethyl)-aminomethane
Notes
Acknowledgement
We would like to thank Tobias Schneider for his support. The authors acknowledge the financial support from Manfred Roth foundation. We would like to thank the University of Salzburg and the Austrian Agency for International Cooperation in Education and Research (OeAD-GmbH) for the scholarship and financial support of Mr. Clemens Gögele.
References
- 1.Delcroix GJ, Kaimrajh DN, Baria D, Cooper S, Reiner T, Latta L et al (2013) Histologic, biomechanical, and biological evaluation of fan-folded iliotibial band allografts for anterior cruciate ligament reconstruction. Arthroscopy 29:756–765CrossRefPubMedGoogle Scholar
- 2.Hu R, Ren YJ, Yan L, Xiao ZH, Ding F, Li F et al (2015) A free anterolateral thigh flap and iliotibial band for reconstruction of soft tissue defects at children's feet and ankles. Injury 46:50–67Google Scholar
- 3.Schulze-Tanzil G, Al-Sadi O, Ertel W, Lohan A (2012) Decellularized tendon extracellular matrix-a valuable approach for tendon reconstruction? Cell 1:1010–1028CrossRefGoogle Scholar
- 4.Huang Z, Godkin O, Schulze-Tanzil G (2016) The challenge in using mesenchymal stromal cells for recellularization of decellularized cartilage. Stem Cell Rev 13:50–67CrossRefGoogle Scholar
- 5.Lohan A, Stoll C, Albrecht M, Denner A, John T, Kruger K et al (2013) Human hamstring tenocytes survive when seeded into a decellularized porcine Achilles tendon extracellular matrix. Connect Tissue Res 54:305–312CrossRefPubMedGoogle Scholar
- 6.Zhao Huang BK, Kokozidou M, Arens S, Schulze-Tanzil G (2016) Establishment of a cytocompatible cell-free intervertebral disc matrix for chondrogenesis with human bone marrow mesenchymal stromal cells. Cells Tissues Organs 201:354–365CrossRefPubMedGoogle Scholar
- 7.Hammer N, Huster D, Boldt A, Hadrich C, Koch H, Mobius R et al (2016) A preliminary technical study on sodium dodecyl sulfate-induced changes of the nano-structural and macro-mechanical properties in human iliotibial tract specimens. J Mech Behav Biomed Mater 61:164–173CrossRefPubMedGoogle Scholar
- 8.Gaspar D, Spanoudes K, Holladay C, Pandit A, Zeugolis D (2015) Progress in cell-based therapies for tendon repair. Adv Drug Deliv Rev 84:240–256CrossRefPubMedGoogle Scholar
- 9.Nooeaid P, Schulze-Tanzil G, Boccaccini AR (2015) Stratified scaffolds for osteochondral tissue engineering. Methods Mol Biol 1340:191–200CrossRefPubMedGoogle Scholar
- 10.Vitagliano L, Berisio R, Mazzarella L, Zagari A (2001) Structural bases of collagen stabilization induced by proline hydroxylation. Biopolymers 58:459–464CrossRefPubMedGoogle Scholar