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Sphingosine-1-Phosphate (S1P) Signaling in Neural Progenitors
Sphingosine-1-phosphate (S1P) and its receptors are important in nervous system development. Reliable in vitro human model systems are needed to further define specific roles for S1P signaling in neural development. We have described S1P-regulated signaling, survival, and differentiation in a human embryonic stem cell-derived neuroepithelial progenitor cell line (hNP1) that expresses functional S1P receptors. These cells can be further differentiated to a neuronal cell type and therefore represent a good model system to study the role of S1P signaling in human neural development. The following sections describe in detail the culture and differentiation of hNP1 cells and two assays to measure S1P signaling in these cells.
Keywords:Adenylyl cyclase cAMP Differentiation G-protein-coupled receptor Inositol phosphates Neural progenitor cells Phospholipase C S1P S1P receptor Second messenger Sphingosine-1-phosphate Stem cells
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