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Preparation of Scaffolds from Decellularized Testicular Matrix

Protocol
Part of the Methods in Molecular Biology book series

Abstract

Biological scaffolds composed of extracellular matrix (ECM) are typically derived by processes that involve decellularization of tissues or organs. Here we describe a simple and robust methodology for the preparation of decellularized testicular matrix (DTM) scaffolds with minimal damage to the native three-dimensional structure and tissue-specific ECM components. Such DTM scaffolds can help to gain a better insight into the molecular composition and function of testicular ECM and to develop new tissue engineering approaches to treat various types of male fertility disorders.

Keywords:

Testis Extracellular matrix Decellularization Scaffold In vitro spermatogenesis 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Biology of the Testis, Research Laboratory for Reproduction, Genetics and Regenerative MedicineVrije Universiteit Brussel (VUB)BrusselsBelgium

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