pp 1-8 | Cite as

A Protocol to Prepare Decellularized Stem Cell Matrix for Rejuvenation of Cell Expansion and Cartilage Regeneration

  • Jingting Li
  • Ming Pei
Part of the Methods in Molecular Biology book series


Traditional ex vivo expansion of adult stem cells yields an insufficient quantity of less potent cells. Here we describe the fabrication of decellularized matrix deposited by synovium-derived stem cells (SDSCs). This matrix could serve as a three-dimensional expansion system to rejuvenate cells for proliferation and tissue-specific differentiation potential, which could benefit cartilage regeneration. The decellularized stem cell matrix (DSCM) might be a powerful system for tissue engineering and regeneration.


Decellularized stem cell matrix Cell expansion Synovium-derived stem cells Chondrogenesis Cartilage tissue engineering Cartilage regeneration 



We thank Suzanne Danley for editing the manuscript. This project was partially supported by Research Grants from the Musculoskeletal Transplant Foundation (MTF) and the National Institutes of Health (AR062763-01A1 and AR067747-01A1).


  1. 1.
    Karnes J, Zhang Y, Pei M (2014) Cell therapy for the creation of cartilage and related clinical trials. In: Templeton NS (ed) Gene and cell therapy: therapeutic mechanisms and strategies, 4th edn. Taylor & Francis, CRC Press, pp 1123–1135Google Scholar
  2. 2.
    Li JT, Pei M (2012) Cell senescence: a challenge in cartilage engineering and regeneration. Tissue Eng Part B Rev 18:270–287CrossRefPubMedGoogle Scholar
  3. 3.
    Pei M, Li JT, Shoukry M, Zhang Y (2011) A review of decellularized stem cell matrix: a novel cell expansion system for cartilage tissue engineering. Eur Cell Mater 22:333–343CrossRefPubMedGoogle Scholar
  4. 4.
    Jones B, Pei M (2012) Synovium-derived stem cells: a tissue-specific stem cell for cartilage engineering and regeneration. Tissue Eng Part B Rev 18:301–311CrossRefPubMedGoogle Scholar
  5. 5.
    Pizzute T, Lynch K, Pei M (2015) Impact of tissue-specific stem cells on lineage specific differentiation: a focus on musculoskeletal system. Stem Cell Rev 11:119–132CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Pei M (2017) Environmental preconditioning rejuvenates stem cells’ chondrogenic potential. Biomaterials 117:10–23CrossRefPubMedGoogle Scholar
  7. 7.
    He F, Chen X, Pei M (2009) Reconstruction of an in vitro tissue-specific microenvironment to rejuvenate synovium-derived stem cells for cartilage tissue engineering. Tissue Eng Part A 15:3809–3821CrossRefPubMedGoogle Scholar
  8. 8.
    Li JT, Pei M (2011) Optimization of an in vitro three-dimensional microenvironment to reprogram synovium-derived stem cells for cartilage tissue engineering. Tissue Eng Part A 17:703–712CrossRefPubMedGoogle Scholar
  9. 9.
    Li J, He F, Pei M (2011) Creation of an in vitro microenvironment to enhance human fetal synovium-derived stem cell chondrogenesis. Cell Tissue Res 345:357–365CrossRefPubMedGoogle Scholar
  10. 10.
    Pei M, Zhang Y, Li JT, Chen DQ (2013) Antioxidation of decellularized stem cell matrix promotes human synovium-derived stem cell-based chondrogenesis. Stem Cells Dev 22:889–900CrossRefPubMedGoogle Scholar
  11. 11.
    Pei M, He F, Li J, Tidwell JE, Jones AC, McDonough EB (2013) Repair of large animal partial-thickness cartilage defects through intraarticular injection matrix-rejuvenated synovium-derived stem cells. Tissue Eng Part A 19:1144–1154CrossRefPubMedGoogle Scholar
  12. 12.
    Lai Y, Sun Y, Skinner CM, Son EL, Lu Z, Tuan RS, Jilka RL, Ling J, Chen XD (2010) Reconstitution of marrow-derived extracellular matrix ex vivo: a robust culture system for expanding large-scale highly functional human mesenchymal stem cells. Stem Cells Dev 19:1095–1107CrossRefPubMedGoogle Scholar
  13. 13.
    Pei M, He F, Kish VL (2011) Expansion on extracellular matrix deposited by human bone marrow stromal cells facilitates stem cell proliferation and tissue-specific lineage potential. Tissue Eng Part A 17:3067–3076CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    He F, Pei M (2013) Extracellular matrix enhances differentiation of adipose stem cells from infrapatellar fat pad toward chondrogenesis. J Tissue Eng Regen Med 7:73–84CrossRefPubMedGoogle Scholar
  15. 15.
    Pei M, He F (2012) Extracellular matrix deposited by synovium-derived stem cells delays replicative senescent chondrocyte dedifferentiation and enhances redifferentiation. J Cell Physiol 227:2163–2174CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    He F, Pei M (2012) Rejuvenation of nucleus pulposus cells using extracellular matrix deposited by synovium-derived stem cells. Spine (Phila Pa 1976) 37:459–469CrossRefGoogle Scholar
  17. 17.
    Pei M, Shoukry M, Li JT, Daffner S, France J, Emery SE (2012) Modulation of in vitro microenvironment facilitates synovium-derived stem cell-based nucleus pulposus tissue regeneration. Spine 37:1538–1547CrossRefPubMedGoogle Scholar
  18. 18.
    Li J, Hansen KC, Zhang Y, Dong C, Dinu CZ, Dzieciatkowska M, Pei M (2014) Rejuvenation of chondrogenic potential in a young stem cell microenvironment. Biomaterials 35:642–653CrossRefPubMedGoogle Scholar
  19. 19.
    Pizzute T, Zhang Y, He F, Pei M (2016) Ascorbate-dependent impact on cell-derived matrix in modulation of stiffness and rejuvenation of infrapatellar fat derived stem cells toward chondrogenesis. Biomed Mater 11:045009ADSCrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, Division of Exercise Physiology, and Mechanical and Aerospace EngineeringWest Virginia UniversityMorgantownUSA
  2. 2.Stem Cell and Tissue Engineering Laboratory, Department of OrthopaedicsWest Virginia UniversityMorgantownUSA

Personalised recommendations