Abstract
Both PINK1 and parkin are the responsible genes (PARK6 and PARK2, respectively) for familial early-onset Parkinson’s disease (PD). Several lines of evidences have suggested that mitochondrial dysfunction would be associated with PD pathogenesis. Lewy body, one of PD pathological hallmarks, contains alpha-synuclein, a familial PD (PARK1/4)-gene product, which is eliminated by macroautophagy, while PINK1 and parkin coordinately mediate mitophagy (hereafter called as PINK1/parkin-mediated mitophagy) reported firstly by Youle’s group. The mitochondrial quality control system is specific for elimination of damaged mitochondria especially in the loss of mitochondrial membrane potential induced by treatment with mitochondrial uncoupler like CCCP or FCCP. In this chapter, we summarized immunocytochemical methods to monitor the PINK1/parkin-mediated mitophagy using cultured cells.
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Acknowledgment
We are grateful for the Grant-in-Aid for Principle Area (S.S., 25111007), the Grant-in-Aid for Scientific Research (B) (S.S., 15H04843), the Grant-in-Aid for Challenging Exploratory Research (S.S., 24659435), the Grant-in-Aid for JSPS Research Fellow (Y.S., 16 J40133), the Grant-in-Aid for Young Scientists (B) (K.I., JP16K19524), and the Grant (Y.I. JP16H00625) from Japan Society for the Promotion of Science and Subsidies to Private Schools.
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The authors declare that they have no competing financial interests.
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Fujimaki, M. et al. (2017). Immunocytochemical Monitoring of PINK1/Parkin-Mediated Mitophagy in Cultured Cells. In: Hattori, N., Saiki, S. (eds) Mitophagy. Methods in Molecular Biology, vol 1759. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_20
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DOI: https://doi.org/10.1007/7651_2017_20
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