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Monitoring Mitophagy During Aging in Caenorhabditis elegans

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Book cover Mitophagy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1759))

Abstract

Mitochondria constitute the main energy-producing centers of eukaryotic cells. In addition, they are involved in several crucial cellular processes, such as lipid metabolism, calcium buffering, and apoptosis. As such, their malfunction can be detrimental for proper cellular physiology and homeostasis. Mitophagy is a mechanism that protects and maintains cellular function by sequestering harmful or dysfunctional mitochondria to lysosomes for degradation. In this report, we present experimental procedures for quantitative, in vivo monitoring of mitophagy events in the nematode Caenorhabditis elegans.

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Acknowledgments

Work in the authors’ laboratory is funded by grants from the European Research Council (ERC), the European Commission Framework Programmes, and the Greek Ministry of Education. Konstantinos Kounakis is a recipient of an Onassis Foundation postgraduate scholarship.

Author information

Author notes

  1. *The authors Nikolaos Charmpilas and Konstantinos Kounakis contributed equally to this chapter.

Authors and Affiliations

  1. Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Vassilika Vouton, Heraklion, Crete, Greece

    Nikolaos Charmpilas, Konstantinos Kounakis & Nektarios Tavernarakis

  2. Department of Biology, University of Crete, Heraklion, 70013, Crete, Greece

    Nikolaos Charmpilas & Konstantinos Kounakis

  3. Department of Basic Sciences, Faculty of Medicine, University of Crete, Heraklion, 71305, Greece

    Nektarios Tavernarakis

Authors
  1. Nikolaos Charmpilas
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  2. Konstantinos Kounakis
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  3. Nektarios Tavernarakis
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Corresponding author

Correspondence to Nektarios Tavernarakis .

Editor information

Editors and Affiliations

  1. Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan

    Nobutaka Hattori

  2. Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan

    Shinji Saiki

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Charmpilas, N., Kounakis, K., Tavernarakis, N. (2017). Monitoring Mitophagy During Aging in Caenorhabditis elegans . In: Hattori, N., Saiki, S. (eds) Mitophagy. Methods in Molecular Biology, vol 1759. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_18

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  • DOI: https://doi.org/10.1007/7651_2017_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7749-9

  • Online ISBN: 978-1-4939-7750-5

  • eBook Packages: Springer Protocols

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