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Mitophagy pp 125–132Cite as

Monitoring of Atg5-Independent Mitophagy

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1759))

Abstract

Mitophagy is a mitochondrial quality control mechanism where damaged and surplus mitochondria are removed by autophagy. There are at least two different mammalian autophagy pathways: the Atg5-dependent conventional pathway and an Atg5-independent alternative pathway; the latter is involved in the erythrocyte mitophagy. In this chapter we describe the various experimental approaches to assess Atg5-indepedndent mitophagy in mammalian cells.

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Authors and Affiliations

  1. Department of Pathological Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan

    Satoko Arakawa, Shinya Honda, Satoru Torii, Masatsune Tsujioka & Shigeomi Shimizu

Authors
  1. Satoko Arakawa
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  2. Shinya Honda
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  3. Satoru Torii
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  4. Masatsune Tsujioka
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  5. Shigeomi Shimizu
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Corresponding author

Correspondence to Shigeomi Shimizu .

Editor information

Editors and Affiliations

  1. Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan

    Nobutaka Hattori

  2. Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan

    Shinji Saiki

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Arakawa, S., Honda, S., Torii, S., Tsujioka, M., Shimizu, S. (2017). Monitoring of Atg5-Independent Mitophagy. In: Hattori, N., Saiki, S. (eds) Mitophagy. Methods in Molecular Biology, vol 1759. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_16

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  • DOI: https://doi.org/10.1007/7651_2017_16

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7749-9

  • Online ISBN: 978-1-4939-7750-5

  • eBook Packages: Springer Protocols

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