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In Vitro Culture of Human Hematopoietic Stem Cells in Serum Free Medium and Their Monitoring by Flow Cytometry

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Stem Cell Heterogeneity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1516))

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Abstract

Hematopoietic stem cells can be isolated from human blood cells trapped in leukoreduction systems. The leukoreduction systems filters or chambers are usually discarded from routine blood or platelet donations in blood banks around the world. These CD34+ cells are a good source of normal stem cells and can be used as models to characterize the blood stem cells before and after culture in vitro. This chapter contains detailed methodologies for the isolation of stem cells from peripheral blood, the culture of these cells in a medium exempt of animal proteins and for the flow cytometry analysis of the resulting cell population for the characterization of their differentiation

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Acknowledgement

C.J. owned a BMP-Innovation-Research Scholarship in a practice environment.

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Authors and Affiliations

  1. Recherche et Développement, Héma-Québec, 1070 avenue des Sciences-de-la-vie, Québec, QC, Canada, G1V 5C3

    Marc Cloutier, Christine Jobin, Carl Simard & Sonia Néron Ph.D.

  2. Faculté des sciences et de génie, Biochimie, Microbiologie et Bio-informatique, Université Laval, Québec, QC, Canada

    Christine Jobin & Sonia Néron Ph.D.

Authors
  1. Marc Cloutier
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  2. Christine Jobin
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  3. Carl Simard
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  4. Sonia Néron Ph.D.
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Corresponding author

Correspondence to Sonia Néron Ph.D. .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2016 Springer Science+Business Media New York

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Cloutier, M., Jobin, C., Simard, C., Néron, S. (2016). In Vitro Culture of Human Hematopoietic Stem Cells in Serum Free Medium and Their Monitoring by Flow Cytometry. In: Turksen, K. (eds) Stem Cell Heterogeneity. Methods in Molecular Biology, vol 1516. Humana Press, New York, NY. https://doi.org/10.1007/7651_2016_329

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  • DOI: https://doi.org/10.1007/7651_2016_329

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6549-6

  • Online ISBN: 978-1-4939-6550-2

  • eBook Packages: Springer Protocols

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