Abstract
Inner ear hair cells (HCs) function as the primary transducers for perception of sound and balance, while a defect in their formation or their loss results in sensory deficits. In mammals, once HCs are lost, they are not regenerated, and thus, various medical strategies have been proposed for their reproduction. Although embryonic stem (ES) cells are a promising source for cell therapy, little is known about efficient generation of hair cell-like cells (HCLs) from mouse ES cells. In the present protocol, we describe a simple method for obtaining ES-derived murine HCLs (HIST2 method).
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References
Raphael Y (2002) Cochlear pathology, sensory cell death and regeneration. Br Med Bull 63:25–38
Fekete DM (1996) Cell fate specification in the inner ear. Curr Opin Neurobiol 6(4):533–541, doi: S0959-4388(96)80061-4 [pii]
Carey J, Amin N (2006) Evolutionary changes in the cochlea and labyrinth: solving the problem of sound transmission to the balance organs of the inner ear. Anat Rec A: Discov Mol Cell Evol Biol 288(4):482–489. doi:10.1002/ar.a.20306
Wobus AM (2001) Potential of embryonic stem cells. Mol Aspects Med 22(3):149–164, doi: S0098-2997(01)00006-1 [pii]
Wobus AM, Boheler KR (2005) Embryonic stem cells: prospects for developmental biology and cell therapy. Physiol Rev 85(2):635–678. doi:10.1152/physrev.00054.2003, 85/2/635 [pii]
De Silva MG, Hildebrand MS, Christopoulos H, Newman MR, Bell K, Ritchie M, Smyth GK, Dahl HH (2006) Gene expression changes during step-wise differentiation of embryonic stem cells along the inner ear hair cell pathway. Acta Otolaryngol 126(11):1148–1157. doi:10.1080/00016480600702118, Q1L63216X1671V13 [pii]
Rivolta MN, Li H, Heller S (2006) Generation of inner ear cell types from embryonic stem cells. Methods Mol Biol 330:71–92. doi:10.1385/1-59745-036-7:71
Oshima K, Shin K, Diensthuber M, Peng AW, Ricci AJ, Heller S (2010) Mechanosensitive hair cell-like cells from embryonic and induced pluripotent stem cells. Cell 141(4):704–716. doi:10.1016/j.cell.2010.03.035, S0092-8674(10)00353-3 [pii]
Ouji Y, Ishizaka S, Nakamura-Uchiyama F, Yoshikawa M (2012) In vitro differentiation of mouse embryonic stem cells into inner ear hair cell-like cells using stromal cell conditioned medium. Cell Death Dis 3, e314. doi:10.1038/cddis.2012.56
Niwa H, Miyazaki J, Smith AG (2000) Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat Genet 24(4):372–376. doi:10.1038/74199
Nishimura F, Yoshikawa M, Kanda S, Nonaka M, Yokota H, Shiroi A, Nakase H, Hirabayashi H, Ouji Y, Birumachi J, Ishizaka S, Sakaki T (2003) Potential use of embryonic stem cells for the treatment of mouse parkinsonian models: improved behavior by transplantation of in vitro differentiated dopaminergic neurons from embryonic stem cells. Stem Cells 21(2):171–180. doi:10.1634/stemcells.21-2-171
Keller GM (1995) In vitro differentiation of embryonic stem cells. Curr Opin Cell Biol 7(6):862–869, doi:0955-0674(95)80071-9 [pii]
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Yoshikawa, M., Ouji, Y. (2016). Induction of Inner Ear Hair Cells from Mouse Embryonic Stem Cells In Vitro. In: Turksen, K. (eds) Stem Cell Heterogeneity. Methods in Molecular Biology, vol 1516. Humana Press, New York, NY. https://doi.org/10.1007/7651_2016_328
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DOI: https://doi.org/10.1007/7651_2016_328
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