Abstract
CD49f+ CD34+ cells, a population rich in skin epithelial stem cells (EpSCs), were obtained from adult mouse skin and cultured with Wnt-3a for 10 days. On day 10, CD49f+ CD34+ cells were sorted and subjected to a second 10-day culture with Wnt-3a. The same procedures were repeated until fifteenth 10-day culture. CD49f+ CD34+ cells obtained from each 10-day culture retained the same EpSC-characteristics as seen in the original EpSCs from adult mouse skin. Here, wedescribe the culture protocol using Wnt-3a for successful maintenance of EpSCs.
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Ouji, Y., Yoshikawa, M. (2016). Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro. In: Turksen, K. (eds) Stem Cell Heterogeneity. Methods in Molecular Biology, vol 1516. Humana Press, New York, NY. https://doi.org/10.1007/7651_2016_320
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DOI: https://doi.org/10.1007/7651_2016_320
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6549-6
Online ISBN: 978-1-4939-6550-2
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