Skip to main content
SpringerLink
Log in

Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix

  • Protocol
  • First Online:
Organoids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1576))

Abstract

A novel lingual epithelial organoid culture system using a three-dimensional (3D) matrix and growth factors has recently been established. In the culture system, organoids with multilayered squamous keratinized epithelium and typical morphological features of filiform papillae are generated from single lingual epithelial cells at a high efficiency. The culture system is created in order to observe the differentiation and maturation process of each lingual epithelial stem cell and to observe abnormal organoid formation from malignant cells obtained from carcinogen-treated mice. Thus, our culture system will contribute to the advancement of research into the regulatory mechanism of lingual epithelium and the underlying mechanisms of carcinogenesis.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 139.00
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Hume WJ (1986) Kinetics of cell replacement in the stratum granulosum of mouse tongue epithelium. Cell Tissue Kinet 19:195–203

    CAS  PubMed  Google Scholar 

  2. Li F, Cao J, Zhou M (2012) NTPDase 2+ cells generate lingual epithelia and papillae. Front Genet 3:255. doi:https://doi.org/10.3389/fgene.2012.00255

    Article  PubMed  PubMed Central  Google Scholar 

  3. Howard JM, Nuguid JM, Ngole D, Nguyen H (2014) Tcf3 expression marks both stem and progenitor cells in multiple epithelia. Development 141:3143–3152

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Tanaka T, Komai Y, Tokuyama Y, Yanai H, Ohe S, Okazaki K, Ueno H (2013) Identification of stem cells that maintain and regenerate lingual keratinized epithelial cells. Nat Cell Biol 15:511–518

    Article  CAS  PubMed  Google Scholar 

  5. Mbiene JP, Maccallum DK, Mistretta CM (1997) Organ cultures of embryonic rat tongue support tongue and gustatory papilla morphogenesis in vitro without intact sensory ganglia. J Comp Neurol 377:324–340

    Article  CAS  PubMed  Google Scholar 

  6. Zhou Y, Liu HX, Mistretta CM (2006) Bone morphogenic proteins and noggin: inhibiting and inducing fungiform taste papilla development. Dev Biol 297:198–213

    Article  CAS  PubMed  Google Scholar 

  7. Ookura T, Kawamoto K, Tsuzaki H, Mikami Y, Ito Y, Oh SH, Hino A (2002) Fibroblast and epidermal growth factors modulate proliferation and neural cell adhesion molecule expression in epithelial cells derived from the adult mouse tongue. In Vitro Cell Dev Biol Anim 38:365–372

    Article  CAS  PubMed  Google Scholar 

  8. Luo X, Okubo T, Randell S, Hogan BLM (2009) Culture of endodermal stem/progenitor cells of the mouse tongue. In Vitro Cell Dev Biol Anim 45:44–54

    Article  CAS  PubMed  Google Scholar 

  9. Hisha H, Tanaka T, Kanno S, Tokuyama Y, Komai Y, Ohe S, Yanai H, Omachi T, Ueno H (2013) Establishment of a novel lingual organoid culture system: generation of organoids having mature keratinized epithelium from adult epithelial stem cells. Sci Rep 3:3224. doi:https://doi.org/10.1038/srep03224

    Article  PubMed  PubMed Central  Google Scholar 

  10. Flint N, Cove FL, Evans GS (1991) A low-temperature method for the isolation of small-intestinal epithelium along the crypt-villus axis. Biochem J 280:331–334

    Article  PubMed  PubMed Central  Google Scholar 

  11. Red-Horse K, Ueno H, Weissman IL, Krasnow M (2010) Coronary arteries form by developmental reprogramming of venous cells. Nature 464:549–553

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Rinkevich Y, Lindau P, Ueno H, Longaker MT, Weissman IL (2011) Germ-layer and lineage-restricted stem/progenitors regenerate the mouse digit tip. Nature 476:409–413

    Article  CAS  PubMed  Google Scholar 

  13. Tang X-H, Knudsen B, Bemis D, Tickoo S, Gudas LJ (2004) Oral cavity and esophageal carcinogenesis modeled in carcinogen-treated mice. Clin Cancer Res 10:301–313

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgement

This work was financially supported by the Funding Program for Next Generation World-Leading Researchers, The Mochida Memorial Foundation, The Naito Memorial Foundation, The Cell Science Research Foundation, The Uehara Memorial Foundation, The Mitsubishi Foundation, and The Yasuda Memorial Foundation to H.U., and a grant from a Grant-in-aid for Scientific Research (C) 23590953 to H.H.

Author information

Authors and Affiliations

  1. Kansai Medical University, Osaka, Japan

    Hiroko Hisha & Hiroo Ueno

Authors
  1. Hiroko Hisha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hiroo Ueno
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hiroo Ueno .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer Science+Business Media New York

About this protocol

Cite this protocol

Hisha, H., Ueno, H. (2016). Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix. In: Turksen, K. (eds) Organoids. Methods in Molecular Biology, vol 1576. Humana, New York, NY. https://doi.org/10.1007/7651_2016_3

Download citation

  • DOI: https://doi.org/10.1007/7651_2016_3

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7616-4

  • Online ISBN: 978-1-4939-7617-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation