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Organoids pp 55–92Cite as

Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1576))

Abstract

Induced pluripotent stem (iPS) cells are the product of adult somatic cell reprogramming to an embryonic-like state by inducing a “forced” expression of specific genes. They are similar to natural pluripotent stem cells, such as embryonic stem (ES) cells, in many aspects, such as the expression of certain stem cell genes and potency and differentiability. Human iPS cells are invaluable resource for basic research, cell therapy, drug discovery, and human organ tissue engineering. iPS cells can be derived from the patient to be treated and thus are genetically identical cells that may avoid immune rejection. The following protocols offer a general guideline for the induction of iPSCs from fibroblasts, and for culture and expansion to produce lung precursor cells.

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References

  1. Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, Vassena R, Bilić J, Pekarik V, Tiscornia G et al (2008) Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Nat Biotechnol 26(11):1276–1284

    Article  CAS  PubMed  Google Scholar 

  2. BD Biosciences. (2013) Assay methods protocol: human embryonic stem cell culture. https://www.daigger.com:1-7

  3. Ghaedi M, Calle EA, Mendez JJ, Gard AL, Balestrini J, Booth A, Bove PF, Gui L, White ES, Niklason LE (2013) Human iPS cell-derived alveolar epithelium repopulates lung extracellular matrix. J Clin Invest 123(11):4950–4962. doi: https://doi.org/10.1172/JCI68793

  4. Huang SX, Islam MN, O'Neill J, Hu Z, Yang YG, Chen YW, Mumau M, Green MD, Vunjak-Novakovic G, Bhattacharya J et al (2014) Efficient generation of lung and airway epithelial cells from human pluripotent stem cells. Nat Biotechnol 32(1):84–91. doi: https://doi.org/10.1038/nbt.2754

  5. Huangfu D, Maehr R, Guo W, Eijkelenboom A, Snitow M, Chen AE, Melton DA (2008) Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds. Nat Biotechnol 26(7):795–797

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Kim D, Kim CH, Moon JI, Chung YG, Chang MY, Han BS, Ko S, Yang E, Cha KY, Lanza R et al (2009) Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins. Cell Stem Cell 4(6):472–476

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Longmire TA, Ikonomou L, Hawkins F, Christodoulou C, Cao Y, Jean JC, Kwok LW, Mou H, Rajagopal J, Shen SS et al (2012) Efficient derivation of purified lung and thyroid progenitors from embryonic stem cells. Cell Stem Cell 10(4):398–411

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Ludwig TE, Levenstein ME, Jones JM, Berggren WT, Mitchen ER, Frane JL, Crandall LJ, Daigh CA, Conard KR, Piekarczyk MS et al (2006) Derivation of human embryonic stem cells in defined conditions. Nat Biotechnol 24(2):185–187

    Article  CAS  PubMed  Google Scholar 

  9. Stein GS, Borowski M, Luong MX, Shi M-J, Smith KP, Vazquez P (2010) Human embryonic stem cell culture on BD Matrigel™ with mTeSR®1 medium. Human Stem Cell Technol Biol. doi:https://doi.org/10.1002/9780470889909.ch11

    Article  Google Scholar 

  10. Stem Cell Technologies. (2012) Technical manual: maintenance of human pluripotent stem cells in mTeSR™1 and TeSR™2. http://www.stemcell.com:1-24

  11. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131(5):861–872

    Article  CAS  PubMed  Google Scholar 

  12. Van Haute L, De Block G, Liebaers I et al. (2009) Generation of lung epithelial-like tissue from human embryonic stem cells. Respir Res 10:105

    Google Scholar 

  13. Wang D, Haviland DL, Burns AR, Zsigmond E, Wetsel RA (2007) A pure population of lung alveolar epithelial type II cells derived from human embryonic stem cells. Proc Natl Acad Sci U S A 104(11):4449–4454

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hämäläinen R, Cowling R, Wang W, Liu P, Gertsenstein M et al (2009) piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature 458(7239):766–770

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Wong AP, Bear CE, Chin S, Pasceri P, Thompson TO, Huan LJ, Ratjen F, Ellis J, Rossant J (2012) Directed differentiation of human pluripotent stem cells into mature airway epithelia expressing functional CFTRTR protein. Nat Biotechnol 30(9):876–882

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA (2009) Human induced pluripotent stem cells free of vector and transgene sequences. Science 324(5928):797–801

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Departments of Anesthesia and Biomedical Engineering, Yale University, New Haven, CT, 06520, USA

    Mahboobe Ghaedi & Laura E. Niklason

  2. Department of Biomedical Engineering, Yale University, New Haven, CT, 06520, USA

    Mahboobe Ghaedi & Laura E. Niklason

Authors
  1. Mahboobe Ghaedi
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  2. Laura E. Niklason
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Corresponding author

Correspondence to Mahboobe Ghaedi .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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© 2016 Springer Science+Business Media New York

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Ghaedi, M., Niklason, L.E. (2016). Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells. In: Turksen, K. (eds) Organoids. Methods in Molecular Biology, vol 1576. Humana, New York, NY. https://doi.org/10.1007/7651_2016_11

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  • DOI: https://doi.org/10.1007/7651_2016_11

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7616-4

  • Online ISBN: 978-1-4939-7617-1

  • eBook Packages: Springer Protocols

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