Quantitative Analysis of Intra-chromosomal Contacts: The 3C-qPCR Method

  • Vuthy Ea
  • Franck Court
  • Thierry FornéEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1589)


The chromosome conformation capture (3C) technique is fundamental to many population-based methods investigating chromatin dynamics and organization in eukaryotes. Here, we provide a modified quantitative 3C (3C-qPCR) protocol for improved quantitative analyses of intra-chromosomal contacts. We also describe an algorithm for data normalization which allows more accurate comparisons between contact profiles.


Chromosome conformation capture Chromatin dynamics and organization Quantitative PCR 



This work was supported by grants from the Institut National du Cancer [contract N° INCa_5960, PLBIO 2012-129, to T.F.], the Association pour la Recherche contre le Cancer [ARC contract n°SFI20101201555 to T.F.], the Ligue contre le cancer (comité Hérault), and the Centre National de la Recherche Scientifique (CNRS).


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Institut de Génétique Moléculaire de Montpellier, UMR5535, CNRSUniversité de MontpellierMontpellier Cedex 5France
  2. 2.Inserm UMR1103, CNRS UMR6293, F-63001 Clermont-Ferrand, France and Clermont UniversitéUniversité d’Auvergne, Laboratoire GReDClermont-FerrandFrance

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