Abstract
The chromosome conformation capture (3C) technique is fundamental to many population-based methods investigating chromatin dynamics and organization in eukaryotes. Here, we provide a modified quantitative 3C (3C-qPCR) protocol for improved quantitative analyses of intra-chromosomal contacts. We also describe an algorithm for data normalization which allows more accurate comparisons between contact profiles.
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Acknowledgement
This work was supported by grants from the Institut National du Cancer [contract N° INCa_5960, PLBIO 2012-129, to T.F.], the Association pour la Recherche contre le Cancer [ARC contract n°SFI20101201555 to T.F.], the Ligue contre le cancer (comité Hérault), and the Centre National de la Recherche Scientifique (CNRS).
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Ea, V., Court, F., Forné, T. (2015). Quantitative Analysis of Intra-chromosomal Contacts: The 3C-qPCR Method. In: Haggarty, P., Harrison, K. (eds) Population Epigenetics. Methods in Molecular Biology, vol 1589. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_269
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DOI: https://doi.org/10.1007/7651_2015_269
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6901-2
Online ISBN: 978-1-4939-6903-6
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