5-Hydroxymethylcytosine Profiling in Human DNA

  • John P. Thomson
  • Colm E. Nestor
  • Richard R. MeehanEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1589)


Since its “re-discovery” in 2009, there has been significant interest in defining the genome-wide distribution of DNA marked by 5-hydroxymethylation at cytosine bases (5hmC). In recent years, technological advances have resulted in a multitude of unique strategies to map 5hmC across the human genome. Here we discuss the wide range of approaches available to map this modification and describe in detail the affinity based methods which result in the enrichment of 5hmC marked DNA for downstream analysis.


5-hydroxymethylcytosine 5hmC 5mC DNA immunoprecipitation and enrichment HmeDIP Epigenetics 



We thank Dr. Jamie Hackett for his role in initially developing the hmeDIP protocol presented here. Research in RRMs lab is supported by the Medical Research Council, the BBSRC and IMI-MARCAR: the Innovative Medicine Initiative Joint Undertaking (IMI JU) under grant agreement number 115001 (MARCAR project. URL:


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • John P. Thomson
    • 1
  • Colm E. Nestor
    • 2
  • Richard R. Meehan
    • 1
    Email author
  1. 1.MRC Human Genetics Unit, Institute of Genetics and Molecular MedicineThe University of EdinburghEdinburghUK
  2. 2.The Centre for Individualized Medication, Linköping University Hospital, Linköping UniversityLinköpingSweden

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