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Maximizing Clonal Embryonic Stem Cell Derivation by ERK Pathway Inhibition

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Embryonic Stem Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1341))

Abstract

Since the development of inhibitor-based defined culture conditions (known as “2i”), multiple clonal embryonic stem cell (ESC) lines can be readily derived from single cells isolated directly from mouse embryos. In addition to providing an efficient means to generate ES cells from compound transgenic or murine disease models on any genetic background, this technology can be used to investigate the process of ESC derivation at both a functional and molecular level. Here, we provide details of the procedure for both maximizing the number of cells in the donor tissue and subsequent effective derivation of multiple clonal ES cell lines.

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Author information

Authors and Affiliations

  1. Wellcome Trust—Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, UK

    Jennifer Nichols & Thorsten Boroviak

  2. Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, UK

    Jennifer Nichols

Authors
  1. Jennifer Nichols
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  2. Thorsten Boroviak
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Corresponding author

Correspondence to Jennifer Nichols .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2015 Springer Science+Business Media New York

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Nichols, J., Boroviak, T. (2015). Maximizing Clonal Embryonic Stem Cell Derivation by ERK Pathway Inhibition. In: Turksen, K. (eds) Embryonic Stem Cell Protocols. Methods in Molecular Biology, vol 1341. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_253

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  • DOI: https://doi.org/10.1007/7651_2015_253

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2953-5

  • Online ISBN: 978-1-4939-2954-2

  • eBook Packages: Springer Protocols

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