Methods for Ex Vivo Analysis of Immune Cell Function from the Central Nervous System

  • Darryl G. Turner
  • Melanie D. Leech
  • Richard A. O’Connor
  • Stephen M. AndertonEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1304)


Experimental autoimmune encephalomyelitis (EAE) is an animal model commonly used to investigate the inflammatory response in organ-specific autoimmunity and a model of the early immune responses of multiple sclerosis.

This protocol outlines the methods used for the processing of peripheral immune tissues, the spleen and draining lymph nodes, as well as the site of inflammation, the central nervous system (CNS), for analyzing immune cell phenotype and function during murine EAE.


Experimental autoimmune encephalomyelitis CNS Autoimmune disease Inflammatory cells Cytokines Transcription factors T cells 



Work in the authors’ laboratory was supported by grants from the UK Medical Research Council and the Wellcome Trust.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Darryl G. Turner
    • 1
    • 2
  • Melanie D. Leech
    • 1
    • 2
  • Richard A. O’Connor
    • 1
    • 2
  • Stephen M. Anderton
    • 1
    • 2
    Email author
  1. 1.MRC Centre for Inflammation Research, Queen’s Medical Research InstituteUniversity of EdinburghEdinburghUK
  2. 2.Centre for Multiple Sclerosis Research, Queen’s Medical Research InstituteUniversity of EdinburghEdinburghUK

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