Abstract
Obesity and its comorbidity incidence have increased worldwide during the past 10 years. In consequence, researchers have drawn their attention to the understanding of adipocyte differentiation. Several cellular model systems have been established; however no efficient protocol could be developed so far to differentiate the pluripotent embryonic stem cells to adipocytes. In this chapter, we describe a detailed protocol that is optimized for mouse embryonic stem cells. The result of this differentiation is a homogenous adipocyte monolayer culture that can be used for several applications including developmental and pharmacological research.
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Acknowledgments
The authors would like to thank Dr. Gerardo Alvarado for the artwork. L.N. is supported by a grant from the Hungarian Scientific Research Fund (OTKA K100196). L.N. and I.C-M. are recipients of the following grant TÁMOP-4.2.2.A-11/1/KONV-2012-0023 VÉD-ELEM implemented through the New Hungary Development Plan cofinanced by the European Social Fund and the European Regional Development Fund. Z.S. is a recipient of TÁMOP 4.2.4. A/2-11-1-2012-0001/A2-JÁDJ-13.
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Cuaranta-Monroy, I., Simandi, Z., Nagy, L. (2015). Differentiation of Adipocytes in Monolayer from Mouse Embryonic Stem Cells. In: Turksen, K. (eds) Embryonic Stem Cell Protocols. Methods in Molecular Biology, vol 1341. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_219
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DOI: https://doi.org/10.1007/7651_2015_219
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2953-5
Online ISBN: 978-1-4939-2954-2
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