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A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells

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Embryonic Stem Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1341))

Abstract

Human embryonic stem cells hold great promise for future biomedical applications such as disease modeling and regenerative medicine. However, these cells are notoriously difficult to culture and are refractory to common means of genetic manipulation, thereby limiting their range of applications. In this protocol, we present an easy and robust method of gene repression in human embryonic stem cells using lipofection of small interfering RNA (siRNA).

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Acknowledgements

We are grateful to Drs. Constance Ciaudo and Anton Wutz for critical comments on the manuscript.

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Authors and Affiliations

  1. Institute for Molecular Health Sciences, ETH Zurich, Zurich, Switzerland

    Peter F. Renz & Tobias A. Beyer

  2. Molecular Life Science program, Life Science Zurich Graduate School, University of Zürich, Zurich, Switzerland

    Peter F. Renz

  3. Department of Biology, Institute for Molecular Health Sciences, ETH Zurich, HPL E16, Otto-Stern-Weg 7, 8093, Zurich, Switzerland

    Tobias A. Beyer

  4. Lunenfeld-Tanenbaum Research Institute, Toronto, ON, Canada

    Tobias A. Beyer

Authors
  1. Peter F. Renz
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  2. Tobias A. Beyer
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Corresponding author

Correspondence to Tobias A. Beyer .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2015 Springer Science+Business Media New York

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Renz, P.F., Beyer, T.A. (2015). A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells. In: Turksen, K. (eds) Embryonic Stem Cell Protocols. Methods in Molecular Biology, vol 1341. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_212

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  • DOI: https://doi.org/10.1007/7651_2015_212

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2953-5

  • Online ISBN: 978-1-4939-2954-2

  • eBook Packages: Springer Protocols

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