Abstract
Here we have described a procedure to generate embryonic stem cell (ESC) lines from rabbit preimplantation blastocysts. We have also provided detailed procedures to characterize the resulting ESC lines, such as the analysis of pluripotency marker expression by reverse transcription quantitative polymerase chain reaction, immunolabeling, and fluorescence-associated cell sorting; evaluation of pluripotency by teratoma production; and assessment of genetic stability by karyotyping.
*These authors contributed equally to this work
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Acknowledgements
This work was supported by research grants from ANR (project PLURABBIT n°PCS-09-GEM-08), COST Action (RGB-Net n°TD1101), and the HyPharm Company, and by the “Infrastructure Nationale en Biologie et Santé” INGESTEM (ANR-11-INBS-0009), the LabEx “CORTEX” (ANR-11-LABEX-0042) of the University of Lyon within the programme “Investissements d’Avenir” (ANR-11-IDEX-0007), and the IHU-B CESAME (ANR-10-IBHU-003).
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Afanassieff, M., Osteil, P., Savatier, P. (2015). Generation of Embryonic Stem Cells in Rabbits. In: Turksen, K. (eds) Embryonic Stem Cell Protocols. Methods in Molecular Biology, vol 1341. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_209
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DOI: https://doi.org/10.1007/7651_2015_209
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2953-5
Online ISBN: 978-1-4939-2954-2
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