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Multiple Sclerosis pp 93–98Cite as

Neurofilament Light Chain Determination from Peripheral Blood Samples

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1304))

Abstract

The loss of neurological function is closely related to axonal damage. Neurofilament subunits are concentrated in neurons and axons and have emerged as promising biomarkers for neurodegeneration. Electrochemiluminescence (ECL) based assays are known to be of superior sensitivity and require less sample volume than conventional ELISAs. Here, we describe a highly sensitive ECL based immunoassay for quantification of neurofilament light chain (NfL) in blood and CSF.

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Author information

Authors and Affiliations

  1. Neurology, University Hospital Basel, Petersgraben 4, 4031, Basel, Switzerland

    Marguerite Limberg, Christian Barro & Jens Kuhle M.D.

  2. Neurocenter of Southern Switzerland, Ospedale Regionale di Lugano, Lugano, Switzerland

    Giulio Disanto

Authors
  1. Marguerite Limberg
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  2. Giulio Disanto
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  3. Christian Barro
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  4. Jens Kuhle M.D.
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Corresponding author

Correspondence to Jens Kuhle M.D. .

Editor information

Editors and Affiliations

  1. University of Regensburg, Regensburg, Germany

    Robert Weissert

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© 2015 Springer Science+Business Media New York

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Limberg, M., Disanto, G., Barro, C., Kuhle, J. (2015). Neurofilament Light Chain Determination from Peripheral Blood Samples. In: Weissert, R. (eds) Multiple Sclerosis. Methods in Molecular Biology, vol 1304. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_206

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  • DOI: https://doi.org/10.1007/7651_2015_206

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2629-9

  • Online ISBN: 978-1-4939-2630-5

  • eBook Packages: Springer Protocols

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