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Patient-Specific Induced Pluripotent Stem Cell Models pp 285–307Cite as

Generation of Human Induced Pluripotent Stem Cells Using RNA-Based Sendai Virus System and Pluripotency Validation of the Resulting Cell Population

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1353))

Abstract

Human induced pluripotent stem cells (hiPSCs) provide a platform for studying human disease in vitro, increase our understanding of human embryonic development, and provide clinically relevant cell types for transplantation, drug testing, and toxicology studies. Since their discovery, numerous advances have been made in order to eliminate issues such as vector integration into the host genome, low reprogramming efficiency, incomplete reprogramming and acquisition of genomic instabilities. One of the ways to achieve integration-free reprogramming is by using RNA-based Sendai virus. Here we describe a method to generate hiPSCs with Sendai virus in both feeder-free and feeder-dependent culture systems. Additionally, we illustrate methods by which to validate pluripotency of the resulting stem cell population.

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Author information

Authors and Affiliations

  1. Institute of Genetic Medicine, Newcastle University, Newcastle-upon-Tyne, UK

    Valeria Chichagova, Irene Sanchez-Vera, Lyle Armstrong, David Steel & Majlinda Lako

  2. Sunderland Eye Infirmary, Sunderland, UK

    David Steel

Authors
  1. Valeria Chichagova
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  2. Irene Sanchez-Vera
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  3. Lyle Armstrong
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  4. David Steel
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  5. Majlinda Lako
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Corresponding author

Correspondence to Valeria Chichagova .

Editor information

Editors and Affiliations

  1. Lunenfeld-Tanenbaum Research Institute, Toronto, Ontario, Canada

    Andras Nagy

  2. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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Chichagova, V., Sanchez-Vera, I., Armstrong, L., Steel, D., Lako, M. (2015). Generation of Human Induced Pluripotent Stem Cells Using RNA-Based Sendai Virus System and Pluripotency Validation of the Resulting Cell Population. In: Nagy, A., Turksen, K. (eds) Patient-Specific Induced Pluripotent Stem Cell Models. Methods in Molecular Biology, vol 1353. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_205

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  • DOI: https://doi.org/10.1007/7651_2015_205

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3033-3

  • Online ISBN: 978-1-4939-3034-0

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