Experimental Autoimmune Encephalomyelitis in Mice

  • Rachael L. Terry
  • Igal Ifergan
  • Stephen D. MillerEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1304)


Experimental autoimmune encephalitis (EAE), the animal model of multiple sclerosis (MS), has provided significant insight into the mechanisms that initiate and drive autoimmunity. Several central nervous system proteins and peptides have been used to induce disease, in a number of different mouse strains, to model the diverse clinical presentations of MS. In this chapter, we detail the materials and methods used to induce active and adoptive EAE. We focus on disease induction in the SJL/J, C57BL/6, and BALB/c mouse strains, using peptides derived from proteolipid protein, myelin basic protein, and myelin oligodendrocyte glycoprotein. We also include a protocol for the isolation of leukocytes from the spinal cord and brain for flow cytometric analysis.


Experimental autoimmune encephalomyelitis Multiple sclerosis Autoimmune disease Mouse model CD4+ T cells 



Central nervous system


Experimental autoimmune encephalomyelitis


Incomplete Freund’s adjuvant


Myelin basic protein


Myelin oligodendrocyte glycoprotein


Multiple sclerosis


Proteolipid protein


T cell receptor


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Rachael L. Terry
    • 1
  • Igal Ifergan
    • 1
  • Stephen D. Miller
    • 2
    Email author
  1. 1.Department of Microbiology-Immunology and Interdepartmental Immunobiology Center, Feinberg School of MedicineNorthwestern UniversityChicagoUSA
  2. 2.Department of Microbiology-Immunology and Interdepartmental Immunobiology Center, Feinberg School of MedicineNorthwestern UniversityChicagoUSA

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