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High-Throughput Cell Aggregate Culture for Stem Cell Chondrogenesis

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Biomimetics and Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1202))

Abstract

Cell aggregate culture is a widely used, reliable system for promoting chondrogenic differentiation of stem cells. A high-throughput cell pellet culture enables screening of various soluble factors for their effects on stem cell function and chondrogenesis. In this protocol, we report a platform that allows the formation of stem cell aggregates in a 96-well plate format. Specifically, stem cells are centrifuged to form high-density pellets, mimicking mesenchymal condensation. The cell aggregates can be differentiated into chondrocytes when cultured in chondrogenic medium for 4 weeks. Such a technique is compatible for high-throughput screening and can be very useful for optimizing conditions for cartilage tissue engineering.

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Acknowledgment

LQW wants to thank the National Science Foundation for funding support.

Author information

Authors and Affiliations

  1. Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA

    Ahmad E. Abu-Hakmeh

  2. Laboratory for Tissue Engineering and Morphogenesis, Department of Biomedical Engineering, 2147 Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY, 12180, USA

    Leo Q. Wan

Authors
  1. Ahmad E. Abu-Hakmeh
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  2. Leo Q. Wan
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Corresponding author

Correspondence to Leo Q. Wan .

Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering and Department of Medicine, Columbia University, New York, New York, USA

    Gordana Vunjak-Novakovic

  2. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2014 Springer Science+Business Media New York

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Abu-Hakmeh, A.E., Wan, L.Q. (2014). High-Throughput Cell Aggregate Culture for Stem Cell Chondrogenesis. In: Vunjak-Novakovic, G., Turksen, K. (eds) Biomimetics and Stem Cells. Methods in Molecular Biology, vol 1202. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_75

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  • DOI: https://doi.org/10.1007/7651_2014_75

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1331-2

  • Online ISBN: 978-1-4939-1332-9

  • eBook Packages: Springer Protocols

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