Abstract
Methods used to understand the function of a gene/protein are one of the hallmarks of modern molecular genetics. The ability to genetically manipulate bacteria has become a fundamental tool in studying these organisms and while basic cloning has become a routine task in molecular biology laboratories, generating directed mutations can be a daunting task. This chapter describes the method of allelic exchange in Staphylococcus aureus using temperature-sensitive plasmids that have successfully produced a variety of chromosomal mutations, including in-frame deletions, insertion of antibiotic-resistance cassettes, and even single-nucleotide point mutations.
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References
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© 2014 Springer Science+Business Media New York
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Lehman, M.K., Bose, J.L., Bayles, K.W. (2014). Allelic Exchange. In: Bose, J. (eds) The Genetic Manipulation of Staphylococci. Methods in Molecular Biology, vol 1373. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_187
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DOI: https://doi.org/10.1007/7651_2014_187
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3157-6
Online ISBN: 978-1-4939-3158-3
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