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Complementation Plasmids, Inducible Gene-Expression Systems, and Reporters for Staphylococci

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The Genetic Manipulation of Staphylococci

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1373))

Abstract

A cornucopia of methods and molecular tools is available for genetic modification of staphylococci, as shown for at least ten different species to date (Prax et al. Microbiology 159:421–435, 2013). This chapter reviews a number of frequently used vectors for complementation purposes that usually replicate in E. coli and staphylococci and differ in parameters including copy number, mode of replication, and sequence length. Systems for the artificial control of gene expression are described that are modulated by low-molecular-weight effectors such as metal cations, carbohydrates, and antibiotics. Finally, the usefulness of reporter proteins that exhibit enzymatic or autofluorescent characteristics in staphylococci is highlighted.

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Acknowledgements

Work in the author’s lab was supported by grant BE4038/1 of the Deutsche Forschungsgemeinschaft (DFG).

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Author notes

  1. Ralph Bertram

    Present address: , Ernst-Simon-Str. 2-4, 72072, Tübingen, Germany

Authors and Affiliations

  1. Department of Microbial Genetics, Faculty of Science, Interfaculty Institute of Microbiology and Infection Medicine Tübingen (IMIT), University of Tübingen, Waldhäuser Str. 70/8, 72076, Tübingen, Germany

    Ralph Bertram

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  1. Ralph Bertram
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Correspondence to Ralph Bertram .

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Editors and Affiliations

  1. University of Kansas Medical Center, Kansas City, Kansas, USA

    Jeffrey L. Bose

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Bertram, R. (2014). Complementation Plasmids, Inducible Gene-Expression Systems, and Reporters for Staphylococci. In: Bose, J. (eds) The Genetic Manipulation of Staphylococci. Methods in Molecular Biology, vol 1373. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_181

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  • DOI: https://doi.org/10.1007/7651_2014_181

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3157-6

  • Online ISBN: 978-1-4939-3158-3

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