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Experimental Autoimmune Encephalomyelitis in Marmosets

  • S. Anwar JagessarEmail author
  • Karin Dijkman
  • Jordon Dunham
  • Bert A. ‘t Hart
  • Yolanda S. Kap
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1304)

Abstract

Experimental autoimmune encephalomyelitis (EAE) in the common marmoset, a small-bodied Neotropical primate, is a well-known and validated animal model for multiple sclerosis (MS). This model can be used for exploratory research, i.e., investigating the pathogenic mechanisms involved in MS, and applied research, testing the efficacy of new potential drugs.

In this chapter, we will describe a method to induce EAE in the marmoset. In addition, we will explain the most common immunological techniques involved in the marmoset EAE research, namely isolation of mononuclear cells (MNC) from peripheral blood and lymphoid tissue, assaying T cell proliferation by thymidine incorporation, MNC phenotyping by flow cytometry, antibody measurement by ELISA, generation of B cell lines and antigen-specific T cell lines, and assaying cytotoxic T cells.

Keywords

Callithrix jacchus Marmoset Experimental autoimmune encephalomyelitis (EAE) Multiple sclerosis Myelin oligodendrocyte glycoprotein (MOG) Immune profiling Flow cytometry Mononuclear cells Cytotoxicity T cell proliferation 

References

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • S. Anwar Jagessar
    • 1
    Email author
  • Karin Dijkman
    • 1
  • Jordon Dunham
    • 1
  • Bert A. ‘t Hart
    • 1
  • Yolanda S. Kap
    • 1
  1. 1.Department of ImmunobiologyBiomedical Primate Research CentreRijswijkThe Netherlands

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