Neuropathological Techniques to Investigate CNS Pathology in Experimental Autoimmune Encephalomyelitis (EAE)

  • Karin Steinbach
  • Doron MerklerEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1304)


Neuropathological techniques such as conventional and immunohistochemical staining of paraffin-embedded tissue sections are instrumental for identification and characterization of aberrations of organ architecture during human inflammatory disorders of the central nervous system (CNS) as in their animal models. Here we describe step-by-step protocols for tissue processing, sectioning, and conventional and immunohistochemical stainings to display as well as quantify CNS inflammation, demyelination, and neuronal damage in experimental autoimmune encephalomyelitis (EAE), an animal model of Multiple Sclerosis (MS).


Experimental autoimmune encephalomyelitis EAE Multiple sclerosis MS Histology Pathology Neuropathology Central nervous system CNS Myelin Axon Hematoxylin Luxol fast blue Bielschowsky HE LFB PAS Inflammation Autoimmunity 



D.M. is supported by the Swiss National Science Foundation (No. PP00P3_128372 and PP00P3_152928), Swiss MS Society, Klaus Tschira Foundation, and Gebert Rüf Foundation.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Pathology and ImmunologyUniversity of GenevaGenevaSwitzerland
  2. 2.Department of NeuropathologyUniversity Medical Center GöttingenGöttingenGermany

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