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GMP-Grade Human Fetal Liver-Derived Mesenchymal Stem Cells for Clinical Transplantation

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Stem Cells and Good Manufacturing Practices

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1283))

Abstract

Stem cell therapy seems a promising avenue in regenerative medicine. Within various stem cells, mesenchymal stem cells have progressively used for cellular therapy. Because of the age-related decreasing in the frequency and differentiating capacity of adult MSCs, fetal tissues such as fetal liver, lung, pancreas, spleen, etc. have been introduced as an alternative source of MSCs for cellular therapy. On the other hand, using stem cells as advanced therapy medicinal products, must be performed in compliance with cGMP as a quality assurance system to ensure the safety, quality, and identity of cell products during translation from the basic stem cell sciences into clinical cell transplantation. In this chapter the authors have demonstrated the manufacturing of GMP-grade human fetal liver-derived mesenchymal stem cells.

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Acknowledgement

This work was supported by Chronic Diseases Research Center, Endocrinology and metabolism research institute, Tehran University of Medical Sciences. The authors would like to thank Dr. Ramin Heshmat, Dr. Seyed Majid Manavi, Dr. Mohsen khorshidi, Dr. Mohamad Reza Amini, and Shokouh Salimi for their considerable assistance.

Author information

Authors and Affiliations

  1. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, 14114, Iran

    Bagher Larijani & Babak Arjmand

  2. Chronic Diseases Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, 14114, Iran

    Hamid-Reza Aghayan

  3. Brain and Spinal Cord Injury Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Parisa Goodarzi

Authors
  1. Bagher Larijani
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  2. Hamid-Reza Aghayan
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  3. Parisa Goodarzi
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  4. Babak Arjmand
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Corresponding author

Correspondence to Babak Arjmand .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2014 Springer Science+Business Media New York

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Larijani, B., Aghayan, HR., Goodarzi, P., Arjmand, B. (2014). GMP-Grade Human Fetal Liver-Derived Mesenchymal Stem Cells for Clinical Transplantation. In: Turksen, K. (eds) Stem Cells and Good Manufacturing Practices. Methods in Molecular Biology, vol 1283. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_101

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  • DOI: https://doi.org/10.1007/7651_2014_101

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2434-9

  • Online ISBN: 978-1-4939-2435-6

  • eBook Packages: Springer Protocols

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