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Transduction of Murine Embryonic Stem Cells by Magnetic Nanoparticle-Assisted Lentiviral Gene Transfer

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Stem Cell Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1058))

Abstract

Genetic modification of embryonic stem (ES) cells is a valuable technique when combined with cell replacement strategies. Obtaining stable transgene expression and low-cytotoxicity lentiviral transduction of ES cells is advantageous. It has been shown that the efficiency of transfection and transduction approaches can be increased by magnetic nanoparticles (MNPs). Here, we present a protocol for MNP-assisted lentiviral transduction of adherent mouse ES cells. The application of MNPs increased transduction efficiency and provided the opportunity of cell positioning by a magnetic field.

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Acknowledgements

Funding was provided to the junior research group “Magnetic nanoparticles (MNPs)-endothelial cell replacement in injured vessels” by the Ministry of Innovation, Science, Research and Technology of the State of North Rhine-Westphalia (D.W.) and by the German Research Foundation (DFG) within the DFG Research Unit FOR 917 “Magnetic Nanoparticle-based targeting of gene- and cell-based therapies” (K.Z.). We thank Olga Mykhaylyk (TU München) for providing MNPs.

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Authors and Affiliations

  1. Institute of Pharmacology and Toxicology, University of Bonn, Bonn, Germany

    Sarah Rieck & Daniela Wenzel

  2. Institute of Physiology, University of Bonn, Bonn, Germany

    Katrin Zimmermann

Authors
  1. Sarah Rieck
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  2. Katrin Zimmermann
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  3. Daniela Wenzel
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Editor information

Editors and Affiliations

  1. Regenerative Medicine Program, Ottawa Hospital Research Institute Sprott Centre for Stem Cell Research, Ottawa, Canada

    Kursad Turksen

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Rieck, S., Zimmermann, K., Wenzel, D. (2013). Transduction of Murine Embryonic Stem Cells by Magnetic Nanoparticle-Assisted Lentiviral Gene Transfer. In: Turksen, K. (eds) Stem Cell Nanotechnology. Methods in Molecular Biology, vol 1058. Humana Press, Totowa, NJ. https://doi.org/10.1007/7651_2013_6

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  • DOI: https://doi.org/10.1007/7651_2013_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-570-5

  • Online ISBN: 978-1-62703-571-2

  • eBook Packages: Springer Protocols

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