Abstract
Self-assembled peptide nanofibers are versatile materials providing suitable platforms for regenerative medicine applications. This chapter describes the use of peptide nanofibers as extracellular matrix mimetic scaffolds for two-dimensional (2D) and three-dimensional (3D) multipotent stromal cell culture systems and procedures for in vitro experiments using these scaffolds. Preparation of 2D and 3D peptide nanofiber scaffolds and cell culturing procedures are presented as part of in vitro experiments including cell adhesion, viability, and spreading analysis. Analysis of cellular differentiation on peptide nanofiber scaffolds is described through immunocytochemistry, qRT-PCR, and other biochemical experiments towards osteogenic and chondrogenic lineage.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
da Silva Meirelles L, Caplan A, Nardi NB (2008) In search of the in vivo identity of mesenchymal stem cells. Stem Cells 26:2287–2299
Crisan M, Yap S, Casteilla L et al (2008) A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell 3:301–313
Saha K, Pollock JF, Schaffer DV, Healy KE (2007) Designing synthetic materials to control stem cell phenotype. Curr Opin Chem Biol 11:381–387
Toksöz S, Guler MO (2009) Self-assembled peptidic nanostructures. Nano Today 4:458–469
Guler MO, Hsu L, Soukasene S, Harrington DA, Hulvat JF, Stupp SI (2006) Presentation of RGDS epitopes on self-assembled nanofibers of branched peptide amphiphiles. Biomacromolecules 7:1855–1863
Ceylan H, Tekinay AB, Guler MO (2011) Selective adhesion and growth of vascular endothelial cells on bioactive peptide nanofiber functionalized stainless steel surface. Biomaterials 32:8797–8805
Ceylan H, Kocabey S, Tekinay AB, Guler MO (2012) Surface-adhesive and osteogenic self-assembled peptide nanofibers for bioinspired functionalization of titanium surfaces. Soft Matter 8:3929–3937
Mammadov B, Mammadov R, Guler MO, Tekinay AB (2012) Cooperative effect of heparan sulfate and laminin mimetic peptide nanofibers on the promotion of neurite outgrowth. Acta Biomater 8:2077–2086
Anderson JM, Kushwaha M, Tambralli A, Bellis SL, Camata RP, Jun H-W (2009) Osteogenic differentiation of human mesenchymal stem cells directed by extracellular matrix-mimicking ligands in a biomimetic self-assembled peptide amphiphile nanomatrix. Biomacromolecules 10:2935–2944
Mata A, Palmer L, Tejeda-Montes E, Stupp SI (2012) Design of biomolecules for nanoengineered biomaterials for regenerative medicine. Methods Mol Biol 811:39–49
Dagdas YS, Tombuloglu A, Tekinay AB, Dana A, Guler MO (2011) Interfiber interactions alter the stiffness of gels formed by supramolecular self-assembled nanofibers. Soft Matter 7:3524–3532
Mammadov R, Tekinay AB, Dana A, Guler MO (2012) Microscopic characterization of peptide nanostructures. Micron 43:69–84
Niece KL, Hartgerink JD, Donners JJJM, Stupp SI (2003) Self-assembly combining two bioactive peptide-amphiphile molecules into nanofibers by electrostatic attraction. J Am Chem Soc 125:7146–7147
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this protocol
Cite this protocol
Ustun, S., Kocabey, S., Guler, M.O., Tekinay, A.B. (2013). Peptide Nanofiber Scaffolds for Multipotent Stromal Cell Culturing. In: Turksen, K. (eds) Stem Cell Nanotechnology. Methods in Molecular Biology, vol 1058. Humana Press, Totowa, NJ. https://doi.org/10.1007/7651_2012_5
Download citation
DOI: https://doi.org/10.1007/7651_2012_5
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-570-5
Online ISBN: 978-1-62703-571-2
eBook Packages: Springer Protocols